SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pennington, J. Articles by Williamson, L. M. Search for Related Content PubMed PubMed Citation Articles by Pennington, J. Articles by Williamson, L. M. Related Collections Transfusion Medicine Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 July 2002, Vol. 100, No. 2, pp. 677-681 TRANSFUSION MEDICINE Persistence of HTLV-I in blood components after leukocyte depletion Joanne Pennington, Graham P. Taylor, Janet Sutherland, Ricardo E. Davis, Jerhard Seghatchian, Jean-Pierre Allain, and Lorna M. Williamson From Department of Haematology, Division of Transfusion Medicine, University of Cambridge; Imperial College/St Mary's Hospital, London; and National Blood Service, East Anglia and Brentwood Centres, United Kingdom. The human T-cell leukemia virus HTLV-I is a transfusion-transmissible retrovirus targeting T lymphocytes for which screening is not currently undertaken in United Kingdom blood donors. The introduction of universal leukocyte depletion (LD) of the United Kingdom blood supply raises the question as to the degree of protection afforded by this procedure against HTLV-I transmission by blood components. HTLV-I viral DNA removal by leukocyte-depleting filters was assessed in units of whole blood and platelets by real-time quantitative polymerase chain reaction (PCR) and by nested PCR for HTLV-I Tax DNA. We examined HTLV-I removal by LD filters using a model system of blood units containing exogenous spiked HTLV-I-positive MT-2 cells at a relevant concentration and whole blood donations from asymptomatic HTLV-I carriers. T-lymphocyte removal was assessed in parallel by measurement of endogenous subset-specific CD3 mRNA. In the MT-2 model system we observed 3.5 log10 to 4 log10 removal of HTLV-I Tax DNA by filtration of whole blood and 2 log10 to 3 log10 removal across platelet filters with 13 of 16 whole blood and 8 of 8 platelet units still positive after filtration. Despite 3 log10 to 4 log10 viral removal, HTLV-I Tax DNA could be detected after whole blood filtration in asymptomatic carriers with viral loads above 108 proviral DNA copies/L. T-lymphocyte removal was also between 3.5 log10 and 4.5 log10. HTLV-I provirus removal was incomplete in the model system and in asymptomatic carriers with viral loads greater than 108 copies/L. These results suggest that LD alone may not provide complete protection from HTLV-I transmission by transfusion. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Leukocyte reduction and HTLV-I: is the glass half empty or half full? Barry Wenz, Girolamo A. Ortolano, Jean-Pierre Allain, and Lorna M. Williamson Blood 2003 101: 370. [Full Text] [PDF] This article has been cited by other articles: M. R. Visconti, J. Pennington, S. F. Garner, J.-P. Allain, and L. M. Williamson Assessment of removal of human cytomegalovirus from blood components by leukocyte depletion filters using real-time quantitative PCR Blood, February 1, 2004; 103(3): 1137 - 1139. [Abstract] [Full Text] [PDF] B. Wenz, G. A. Ortolano, J.-P. Allain, and L. M. Williamson Leukocyte reduction and HTLV-I: is the glass half empty or half full? Blood, January 1, 2003; 101(1): 370 - 370. [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020