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Related Collections Hemostasis, Thrombosis, and Vascular Biology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2002, Vol. 100, No. 3, pp. 1081-1083 BRIEF REPORT Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion János Polgár, Sul-Hee Chung, and Guy L. Reed From the Cardiovascular Biology Laboratory, Harvard School of Public Health; and Massachusetts General Hospital, Boston, MA. Secretion of platelet granules is necessary for normal hemostasis. Platelet secretion requires soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complex formation between different members of the syntaxin, SNAP-25, and vesicle-associated membrane protein (VAMP) gene families. Using microcapillary reverse-phase high-performance liquid chromatography-nano-electrospray tandem mass spectrometry, we identified VAMP-3 and VAMP-8 as VAMP isoforms coimmunoprecipitated from platelets with syntaxin 4. Immunoblotting experiments confirmed the presence of VAMP-3 and VAMP-8 but not VAMP-1 or VAMP-2 in platelets. To examine the effect of VAMP proteins on platelet secretion, soluble recombinant (r) VAMP-2, rVAMP-3, and rVAMP-8 were incubated with streptolysin O-permeabilized platelets. Secretion of  granules (monitored by flow cytometric measurement of P-selectin) was blocked, and dense-granule secretion (assessed by release of carbon 14-serotonin) was almost completely inhibited by rVAMP-3, whereas rVAMP-8 inhibited secretion of dense granules but not  granules. In contrast, rVAMP-2, which formed SNARE complexes in vitro, had no effect on platelet exocytosis. We conclude that VAMP-3 and VAMP-8 form SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. M. Uriarte, D. W. Powell, G. C. Luerman, M. L. Merchant, T. D. Cummins, N. R. Jog, R. A. Ward, and K. R. McLeish Comparison of Proteins Expressed on Secretory Vesicle Membranes and Plasma Membranes of Human Neutrophils J. Immunol., April 15, 2008; 180(8): 5575 - 5581. [Abstract] [Full Text] [PDF] Y. H. S. Ho, D. T. Cai, C.-C. Wang, D. Huang, and S. H. Wong Vesicle-Associated Membrane Protein-8/Endobrevin Negatively Regulates Phagocytosis of Bacteria in Dendritic Cells J. Immunol., March 1, 2008; 180(5): 3148 - 3157. [Abstract] [Full Text] [PDF] D. Shiffman, E. S. O'Meara, L. A. Bare, C. M. Rowland, J. Z. Louie, A. R. Arellano, T. Lumley, K. Rice, O. Iakoubova, M. M. 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	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020