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Related Collections Hemostasis, Thrombosis, and Vascular Biology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2002, Vol. 100, No. 3, pp. 1081-1083 BRIEF REPORT Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion János Polgár, Sul-Hee Chung, and Guy L. Reed From the Cardiovascular Biology Laboratory, Harvard School of Public Health; and Massachusetts General Hospital, Boston, MA. Secretion of platelet granules is necessary for normal hemostasis. Platelet secretion requires soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complex formation between different members of the syntaxin, SNAP-25, and vesicle-associated membrane protein (VAMP) gene families. Using microcapillary reverse-phase high-performance liquid chromatography-nano-electrospray tandem mass spectrometry, we identified VAMP-3 and VAMP-8 as VAMP isoforms coimmunoprecipitated from platelets with syntaxin 4. Immunoblotting experiments confirmed the presence of VAMP-3 and VAMP-8 but not VAMP-1 or VAMP-2 in platelets. To examine the effect of VAMP proteins on platelet secretion, soluble recombinant (r) VAMP-2, rVAMP-3, and rVAMP-8 were incubated with streptolysin O-permeabilized platelets. Secretion of  granules (monitored by flow cytometric measurement of P-selectin) was blocked, and dense-granule secretion (assessed by release of carbon 14-serotonin) was almost completely inhibited by rVAMP-3, whereas rVAMP-8 inhibited secretion of dense granules but not  granules. In contrast, rVAMP-2, which formed SNARE complexes in vitro, had no effect on platelet exocytosis. We conclude that VAMP-3 and VAMP-8 form SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Zhao, L. Yang, J. A. Lopez, J. Fan, J. G. Burchfield, L. Bai, W. Hong, T. Xu, and D. E. James Variations in the requirement for v-SNAREs in GLUT4 trafficking in adipocytes J. Cell Sci., October 1, 2009; 122(19): 3472 - 3480. [Abstract] [Full Text] [PDF] S. Hor, T. Ziv, A. Admon, and P. J. Lehner Stable Isotope Labeling by Amino Acids in Cell Culture and Differential Plasma Membrane Proteome Quantitation Identify New Substrates for the MARCH9 Transmembrane E3 Ligase Mol. Cell. Proteomics, August 1, 2009; 8(8): 1959 - 1971. [Abstract] [Full Text] [PDF] G. J. Graham, Q. Ren, J. R. Dilks, P. Blair, S. W. Whiteheart, and R. 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