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Prepublished online as a Blood First Edition Paper on April 17, 2002; DOI 10.1182/blood-2002-01-0160. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-01-0160v1 100/3/933    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dunne, P. J. Articles by Akbar, A. N. Search for Related Content PubMed PubMed Citation Articles by Dunne, P. J. Articles by Akbar, A. N. Related Collections Immunobiology Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2002, Vol. 100, No. 3, pp. 933-940 IMMUNOBIOLOGY Epstein-Barr virus-specific CD8+ T cells that re-express CD45RA are apoptosis-resistant memory cells that retain replicative potential Padraic J. Dunne, Jeffery M. Faint, Nancy H. Gudgeon, Jean M. Fletcher, Fiona J. Plunkett, Maria Vieira D. Soares, Andrew D. Hislop, Nicola E. Annels, Alan B. Rickinson, Mike Salmon, and Arne N. Akbar From the Department of Clinical Immunology, Royal Free and University College Medical School, London; the Cancer Research Campaign Institute for Cancer Studies, Birmingham University Medical School; and the Medical Research Council Centre for Immune Regulation, Birmingham University Medical School, United Kingdom. During acute infection, latent and lytic Epstein-Barr virus (EBV) epitope-specific CD8+ T cells have a CD45RO+ CD45RA phenotype. However, after resolution of the infection, a large proportion of these cells, particularly those specific for lytic viral epitopes, re-express the CD45RA molecule. The role of CD8+ CD45RA+ T cells in ongoing immunity to EBV and other viruses is unknown. We now demonstrate that, relative to their CD45RO+ counterparts, the EBV-specific CD8+ T cells that revert to CD45RA expression after acute infectious mononucleosis are not in cell cycle, have longer telomeres, and are more resistant to apoptosis partly because of increased Bcl-2 expression. However, the EBV-specific CD8+ CD45RA+ T cells have shorter telomeres than the total CD8+ CD45RA+ T-cell pool and predominantly express low levels of the CCR7 chemokine receptor, indicating that they are not naive cells. In addition, EBV-specific CD8+ CD45RA+ T cells can be induced to proliferate and exhibit potent cytotoxic activity against target cells loaded with specific peptide. Our results strongly suggest, therefore, that EBV-specific CD8+ CD45RA+ T cells represent a stabilized virus-specific memory pool and not terminally differentiated effector cells. The identification of mechanisms that enable stable virus-specific CD8+ T cells to persist after acute infection may lead to the enhancement of antiviral immunity in immunocompromised and elderly persons. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Yuling, X. Ruijing, L. Li, J. Xiang, Z. Rui, W. Yujuan, Z. Lijun, D. Chunxian, T. Xinti, X. Wei, et al. EBV-Induced Human CD8+ NKT Cells Suppress Tumorigenesis by EBV-Associated Malignancies Cancer Res., October 15, 2009; 69(20): 7935 - 7944. 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	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020