SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on June 28, 2002; DOI 10.1182/blood-2001-11-0039. This Article Full Text Full Text (PDF) All Versions of this Article: 2001-11-0039v1 100/4/1484    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lapierre, V. Articles by Tiberghien, P. Search for Related Content PubMed PubMed Citation Articles by Lapierre, V. Articles by Tiberghien, P. Related Collections Transfusion Medicine Transplantation Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2002, Vol. 100, No. 4, pp. 1484-1489 TRANSPLANTATION Increased presence of anti-HLA antibodies early after allogeneic granulocyte colony-stimulating factor-mobilized peripheral blood hematopoietic stem cell transplantation compared with bone marrow transplantation Valérie Lapierre, Anne Aupérin, Hakim Tayebi, Jacqueline Chabod, Philippe Saas, Mauricette Michalet, Sylvie François, Frédéric Garban, Christine Giraud, Dominique Tramalloni, Nadia Oubouzar, Didier Blaise, Matthieu Kuentz, Eric Robinet, and Pierre Tiberghien for the Société Française de Greffe de Moelle et de Thérapie Cellulaire From the Unité de Thérapie Tissulaire, Cellulaire et Génique, and the Laboratoire de Thérapeutique Immuno-Moléculaire, INSERM E 0119/UPRES EA 2284, Etablissement Français du Sang Bourgogne-Franche-Comté, Besançon, France; Service d'Epidémiologie et de Biostatistique and the Unité de Médecine Transfusionnelle et d'Hémovigilance, Institut Gustave Roussy, Villejuif, France; Service d'Hématologie Clinique, Hôpital Edouard Herriot, Lyon, France; Service des Maladies du Sang, Centre Hospitalier Universitaire, Angers, France; Service d'Hématologie Clinique, Centre Hospitalier Universitaire, Grenoble, France; Service d'Hématologie Clinique d'Oncologie et de Thérapie Cellulaire, Centre Hospitalier Universitaire, Etablissement Français du Sang Centre-Atlantique, Poitiers, France; Unité de Transplantation Médullaire, Institut Paoli Calmettes, Marseille, France; and the Service d'Hématologie, Hôpital Henri Mondor, Créteil, France. We have recently shown that the use of allogeneic granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood hematopoietic stem cell transplantation (PBHSCT), as compared with bone marrow transplantation (BMT), is associated with increased titers of antibodies (Abs) directed against red blood cell ABO antigens. To further evaluate the influence of a G-CSF-mobilized PBHSCT graft on alloimmune Ab responses, we examined the frequency of anti-HLA Abs after transplantation in the setting of the same randomized study, comparing PBHSCT with BMT in adults. Anti-HLA Ab presence was determined by complement-dependent cytotoxicity assay (CDC) and flow cytometry in the recipient before and 30 days after transplantation as well as in the donor before graft donation. The use of PBHSCT was significantly associated with increased detection of anti-HLA immunoglobulin G (IgG) Abs early after transplantation as evidenced by flow cytometry (11 of 24 versus 4 of 27 transplant recipients, P = .03) and, less so, by CDC (5 of 24 versus 1 of 27 transplant recipients, P = .09). The difference between PBHSCT and BMT was further heightened when analysis was restricted to anti-HLA IgG Ab-negative donor/recipient pairs. In such a setting, early anti-HLA Ab was never detected after BMT but was repeatedly detected after PBHSCT (flow cytometry, 6 of 18 versus 0 of 17 transplant recipients, P = .02; CDC, 4 of 23 versus 0 of 26 transplant recipients, P = .04). Importantly, the PBHSCT-associated increase in anti-HLA Ab detection was observed despite a reduction in the median number of platelet-transfusion episodes per patient in PBHSC transplant versus BM transplant recipients (3 platelet-transfusion episodes [range, 1-21] in PBHSCT group vs 6 platelet-transfusion episodes [range, 3-33] in the BMT group; P = .02). In conclusion, this study strongly suggests that G-CSF-mobilized PBHSCT results in an increased incidence of circulating anti-HLA Abs and further confirms that the use of such a graft alters alloimmune Ab responses. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Sundin, O. Ringden, B. Sundberg, S. Nava, C. Gotherstrom, and K. Le Blanc No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients Haematologica, September 1, 2007; 92(9): 1208 - 1215. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020