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Blood, 1 September 2002, Vol. 100, No. 5, pp. 1742-1754
IMMUNOBIOLOGY
Identification of the genes differentially expressed in human
dendritic cell subsets by cDNA subtraction and microarray
analysis
Jung Hoon Ahn,
Yoon Lee,
ChoonJu Jeon,
Sang-Jin Lee,
Byung-Hak Lee,
Kang Duk Choi, and
Yong-Soo Bae
From Creagene Research Institute, Creagene,
Tanbang-dong, Seo-gu; and Department of Microbiology, Hannam
University, 133 Ojung-dong, Daedeok-gu, Daejeon, South Korea.
Recent studies on dendritic cell (DC)-associated genes have been
performed using monocyte-derived DCs (MoDCs) in different maturation stages. In our approach, to uncover the novel
DC-associated genes and their expression profiles among the different
DC subsets, we constructed a subtracted DC-cDNA library from
CD1a+, CD14+, and CD11c DCs by
subtracting the genes shared with T cells, B cells, and monocytes, and
we then screened the libraries with the aid of microarray technique.
The genes showing remarkable specificity to DCs in the microarray
analysis were selected and confirmed by semiquantitative reverse
transcriptase-polymerase chain reaction. Our investigations revealed
the following: (1) Genes highly expressed in myeloid DCs are those
involved in antigen uptake/processing/presentation, cell
metamorphosis, or chemotaxis. (2) Most of the genes previously identified in MoDCs, such as TARC, ferritin L-chain, lysosomal acid
lipase,  - and  -tubulin, osteopontin (Eta-1), and others, are not
markedly expressed in CD11c DCs regardless of their
maturation status. On the other hand, specific transcription factors
and MHC class II molecules, such as interferon regulatory factor-4
(IRF4) and HLA-DR, are similarly expressed in both DC subsets. (3)
CD14+ DCs retain unique features of tissue DCs, as
evidenced by the gene expression profile of "no CCR7 but more CCR1"
and "no TARC but abundant MCP1 and Eta-1." (4) The genes for
immunoglobulin (Ig) superfamily Z39Ig, CD20-like precursor,
glycoprotein NMB (GPNMB), transforming growth factor
(TGF-)-induced protein (TGFBI), myeloid DAP12-associated lectin
(MDL-1), and 6 novel genes are newly identified as being associated
with the phenotypic expression of the DC subsets. These identifications
provide important molecular information for further functional studies
of the DC subsets.
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