|
|
Prepublished online as a Blood First Edition Paper on May 31, 2002; DOI 10.1182/blood-2002-03-0949.
 Previous Article | Table of Contents | Next Article 
Blood, 1 October 2002, Vol. 100, No. 7, pp. 2441-2448
HEMATOPOIESIS
Biochemical characterization of PRV-1, a novel hematopoietic cell
surface receptor, which is overexpressed in polycythemia rubra
vera
Steffen Klippel,
Elisabeth Strunck,
Christian E. Busse,
Dirk Behringer, and
Heike L. Pahl
From the Department of Experimental Anaesthesiology,
University Hospital Freiburg, Center for Clinical Research, and the
Department of Hematology and Oncology, University Hospital Freiburg,
Germany.
The cDNA for polycythemia rubra vera 1 (PRV-1), a novel
hematopoietic receptor, was recently cloned by virtue of its
overexpression in patients with polycythemia vera. PRV-1 is a
member of the uPAR/CD59/Ly6 family of cell surface receptors, which
share a common cysteine-rich domain and are tethered to the cell
surface via a glycosylphosphatidylinositol (GPI) link. We have
determined the intron-exon structure of the PRV1
gene and show that the locus is structurally intact in patients with polycythemia vera. Thus, PRV-1 overexpression in these patients is
not due to rearrangement or structural alteration of the gene. Northern
blot analysis detects multiple PRV-1 transcripts. Here we show that
these transcripts arise from alternative polyadenylation and encode the
same protein. Biochemical analysis reveals that PRV-1 is
N-glycosylated and embedded in the cell membrane by a lipid
anchor, like other members of this family. Moreover, PRV-1 is shed from
the cell surface because soluble protein can be detected in cell
supernatants. Fluorescence-activated cell sorting analysis of stably
transfected cells revealed that PRV-1 is recognized by antibodies
directed against the neutrophil antigen NB1/CD177. Flow cytometry of
bone marrow and peripheral blood of both healthy donors and patients
with polycythemia vera showed that PRV-1 protein is expressed on
myeloid cells of the granulocytic lineage. However, unlike the
significant difference in PRV-1 expression observed on the mRNA level,
the amount of PRV-1 protein on the cell surface is not consistently
elevated in patients with polycythemia vera compared with healthy
controls. Therefore, quantification of PRV-1 surface expression cannot
be used for the diagnosis of polycythemia vera.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. von Vietinghoff, G. Tunnemann, C. Eulenberg, M. Wellner, M. Cristina Cardoso, F. C. Luft, and R. Kettritz
NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils
Blood,
May 15, 2007;
109(10):
4487 - 4493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Campbell and A. R. Green
The Myeloproliferative Disorders
N. Engl. J. Med.,
December 7, 2006;
355(23):
2452 - 2466.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Urbanek, D. Torella, F. Sheikh, A. De Angelis, D. Nurzynska, F. Silvestri, C. A. Beltrami, R. Bussani, A. P. Beltrami, F. Quaini, et al.
Myocardial regeneration by activation of multipotent cardiac stem cells in ischemic heart failure
PNAS,
June 14, 2005;
102(24):
8692 - 8697.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Palmqvist, P. Goerttler, C. Wasslavik, P. Johansson, B. Andreasson, S. Safai-Kutti, J. Kutti, H. L. Pahl, and A. Ricksten
Comparison of Methods for Polycythemia Rubra Vera-1 mRNA Quantification in Whole-Blood Leukocytes and Purified Granulocytes
Clin. Chem.,
March 1, 2004;
50(3):
644 - 647.
[Full Text]
[PDF]
|
|
|
|
|
