|
|
Prepublished online as a Blood First Edition Paper on June 21, 2002; DOI 10.1182/blood-2002-03-0902.
 Previous Article | Table of Contents | Next Article 
Blood, 15 October 2002, Vol. 100, No. 8, pp. 2787-2792
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Platelets, circulating tissue factor, and fibrin colocalize in ex
vivo thrombi: real-time fluorescence images of thrombus formation and
propagation under defined flow conditions
Viji Balasubramanian,
Eric Grabowski,
Alessandra Bini, and
Yale Nemerson
From the Division of Thrombosis Research, Mount Sinai
School of Medicine, and the New York Blood Center and Department of
Pathology, Columbia University College of Physicians and Surgeons, New
York, NY; and the Cardiovascular Thrombosis Laboratory, Massachusetts
General HospitalHarvard Medical School, Boston.
Although it is generally accepted that the initial event in
coagulation and intravascular thrombus formation is the exposure of
tissue factor (TF) to blood, there is still little agreement about the
mechanisms of thrombus propagation and the identities of the molecular
species participating in this process. In this study, we characterized
the thrombotic process in real-time and under defined flow conditions
to determine the relative contribution and spatial distribution of 3 components of the thrombi: circulating or blood-borne TF (cTF), fibrin,
and platelets. For this purpose, we used high-sensitivity, multicolor
immunofluorescence microscopy coupled with a laminar flow chamber.
Freshly drawn blood, labeled with mepacrine (marker for platelets and
white cells), anti-hTF1Alexa.568 (marker for tissue
factor), and anti-T2G (marker
for fibrin) was perfused over collagen-coated glass slides at wall
shear rates of 100 and 650 s 1. A motorized filter cube
selector facilitated imaging every 5 seconds at 1 of 3 different
wavelengths, corresponding to optimal wavelengths for the 3 markers
above. Real-time video recordings obtained during each of 10 discrete
experiments show rapid deposition of platelets and fibrin onto
collagen-coated glass. Overlay images of fluorescent markers
corresponding to platelets, fibrin, and cTF clearly demonstrate
colocalization of these 3 components in growing thrombi. These data
further support our earlier observations that, in addition to TF
present in the vessel wall, there is a pool of TF in circulating blood
that contributes to the propagation of thrombosis at a site of vascular injury.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Orfeo, K. E. Brummel-Ziedins, M. Gissel, S. Butenas, and K. G. Mann
The Nature of the Stable Blood Clot Procoagulant Activities
J. Biol. Chem.,
April 11, 2008;
283(15):
9776 - 9786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. T. Nurden
Platelets and Tissue Remodeling: Extending the Role of the Blood Clotting System
Endocrinology,
July 1, 2007;
148(7):
3053 - 3055.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Hathcock
Flow Effects on Coagulation and Thrombosis
Arterioscler Thromb Vasc Biol,
August 1, 2006;
26(8):
1729 - 1737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cho and D. F. Mosher
Enhancement of thrombogenesis by plasma fibronectin cross-linked to fibrin and assembled in platelet thrombi
Blood,
May 1, 2006;
107(9):
3555 - 3563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Touat, V. Ollivier, J. Dai, M.-G. Huisse, A. Bezeaud, U. Sebbag, T. Palombi, P. Rossignol, O. Meilhac, M.-C. Guillin, et al.
Renewal of Mural Thrombus Releases Plasma Markers and Is Involved in Aortic Abdominal Aneurysm Evolution
Am. J. Pathol.,
March 1, 2006;
168(3):
1022 - 1030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Thattaliyath, M. Cykowski, and P. Jagadeeswaran
Young thrombocytes initiate the formation of arterial thrombi in zebrafish
Blood,
July 1, 2005;
106(1):
118 - 124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. S. Coller
Leukocytosis and Ischemic Vascular Disease Morbidity and Mortality: Is It Time to Intervene?
Arterioscler Thromb Vasc Biol,
April 1, 2005;
25(4):
658 - 670.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Butenas, B. A. Bouchard, K. E. Brummel-Ziedins, B. Parhami-Seren, and K. G. Mann
Tissue factor activity in whole blood
Blood,
April 1, 2005;
105(7):
2764 - 2770.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Palmerini, B. S. Coller, V. Cervi, L. Tomasi, A. Marzocchi, C. Marrozzini, O. Leone, M. Piccioli, and A. Branzi
Monocyte-derived tissue factor contributes to stent thrombosis in an in vitro system
J. Am. Coll. Cardiol.,
October 19, 2004;
44(8):
1570 - 1577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Aras, A. Shet, R. R. Bach, J. L. Hysjulien, A. Slungaard, R. P. Hebbel, G. Escolar, B. Jilma, and N. S. Key
Induction of microparticle- and cell-associated intravascular tissue factor in human endotoxemia
Blood,
June 15, 2004;
103(12):
4545 - 4553.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Dietzen, K. L. Page, and T. A. Tetzloff
Lipid rafts are necessary for tonic inhibition of cellular tissue factor procoagulant activity
Blood,
April 15, 2004;
103(8):
3038 - 3044.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Tutar, M. Ozcan, M. Kilickap, S. Gulec, O. Aras, G. Pamir, D. Oral, L. Dandelet, and N. S. Key
Elevated Whole-Blood Tissue Factor Procoagulant Activity as a Marker of Restenosis After Percutaneous Transluminal Coronary Angioplasty and Stent Implantation
Circulation,
September 30, 2003;
108(13):
1581 - 1584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
