|
|
Prepublished online as a Blood First Edition Paper on September 19, 2002; DOI 10.1182/blood-2002-02-0438.
 Previous Article | Table of Contents | Next Article 
Blood, 1 February 2003, Vol. 101, No. 3, pp. 1015-1023
IMMUNOBIOLOGY
The transcription factor Spi-B is expressed in plasmacytoid
DC precursors and inhibits T-, B-, and NK-cell development
Remko Schotte,
Marie-Clotilde Rissoan,
Nathalie Bendriss-Vermare,
Jean-Michel Bridon,
Thomas Duhen,
Kees Weijer,
Francine Brière, and
Hergen Spits
From the Division of Immunology of the Netherlands
Cancer Institute, Amsterdam, The Netherlands; and Schering
Plough Laboratory for Immunological Research, Dardilly,
France.
Human plasmacytoid dendritic cells (pDCs), also called type 2 dendritic cell precursors or natural interferon (IFN)-producing cells,
represent a cell type with distinctive phenotypic and functional features. They are present in the thymus and probably share a common
precursor with T and natural killer (NK) cells. In an effort to
identify genes that control pDC development we searched for genes of
which the expression is restricted to human pDC using a cDNA
subtraction technique with activated monocyte-derived DCs (Mo-DCs) as
competitor. We identified the transcription factor Spi-B to be
expressed in pDCs but not in Mo-DCs. Spi-B expression in pDCs was
maintained on in vitro maturation of pDCs. Spi-B was expressed in
early CD34+CD38 hematopoietic progenitors and
in CD34+CD1a thymic precursors. Spi-B
expression is down-regulated when uncommitted CD34+CD1a thymic precursors differentiate
into committed CD34+CD1a+ pre-T cells.
Overexpression of Spi-B in hematopoietic progenitor cells resulted in
inhibition of development of T cells both in vitro and in vivo. In
addition, development of progenitor cells into B and NK cells in vitro
was also inhibited by Spi-B overexpression. Our results indicate that
Spi-B is involved in the control of pDC development by limiting the
capacity of progenitor cells to develop into other lymphoid lineages.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Lenz, I. Nagel, R. Siebert, A. V. Roschke, W. Sanger, G. W. Wright, S. S. Dave, B. Tan, H. Zhao, A. Rosenwald, et al.
Aberrant immunoglobulin class switch recombination and switch translocations in activated B cell-like diffuse large B cell lymphoma
J. Exp. Med.,
March 19, 2007;
204(3):
633 - 643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Dontje, R. Schotte, T. Cupedo, M. Nagasawa, F. Scheeren, R. Gimeno, H. Spits, and B. Blom
Delta-like1-induced Notch1 signaling regulates the human plasmacytoid dendritic cell versus T-cell lineage decision through control of GATA-3 and Spi-B
Blood,
March 15, 2006;
107(6):
2446 - 2452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Hoyer, M. Herling, K. Bagrintseva, D. W. Dawson, S. W. French, M. Renard, J. G. Weinger, D. Jones, and M. A. Teitell
T Cell Leukemia-1 Modulates TCR Signal Strength and IFN-{gamma} Levels through Phosphatidylinositol 3-Kinase and Protein Kinase C Pathway Activation
J. Immunol.,
July 15, 2005;
175(2):
864 - 873.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Omatsu, T. Iyoda, Y. Kimura, A. Maki, M. Ishimori, N. Toyama-Sorimachi, and K. Inaba
Development of Murine Plasmacytoid Dendritic Cells Defined by Increased Expression of an Inhibitory NK Receptor, Ly49Q
J. Immunol.,
June 1, 2005;
174(11):
6657 - 6662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Pelayo, J. Hirose, J. Huang, K. P. Garrett, A. Delogu, M. Busslinger, and P. W. Kincade
Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow
Blood,
June 1, 2005;
105(11):
4407 - 4415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Lefebvre, M. C. Haks, M. O. Carleton, M. Rhodes, G. Sinnathamby, M. C. Simon, L. C. Eisenlohr, L. A. Garrett-Sinha, and D. L. Wiest
Enforced Expression of Spi-B Reverses T Lineage Commitment and Blocks {beta}-Selection
J. Immunol.,
May 15, 2005;
174(10):
6184 - 6194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Garnache-Ottou, L. Chaperot, S. Biichle, C. Ferrand, J.-P. Remy-Martin, E. Deconinck, P. D. de Tailly, B. Bulabois, J. Poulet, E. Kuhlein, et al.
Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells
Blood,
February 1, 2005;
105(3):
1256 - 1264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Nutt, D. Metcalf, A. D'Amico, M. Polli, and L. Wu
Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
J. Exp. Med.,
January 18, 2005;
201(2):
221 - 231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. McKenna, A.-S. Beignon, and N. Bhardwaj
Plasmacytoid Dendritic Cells: Linking Innate and Adaptive Immunity
J. Virol.,
January 1, 2005;
79(1):
17 - 27.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Chicha, D. Jarrossay, and M. G. Manz
Clonal Type I Interferon-producing and Dendritic Cell Precursors Are Contained in Both Human Lymphoid and Myeloid Progenitor Populations
J. Exp. Med.,
December 6, 2004;
200(11):
1519 - 1524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Yui and E. V. Rothenberg
Deranged Early T Cell Development in Immunodeficient Strains of Nonobese Diabetic Mice
J. Immunol.,
November 1, 2004;
173(9):
5381 - 5391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Takeshita, K. Suzuki, S. Sasaki, N. Ishii, D. M. Klinman, and K. J. Ishii
Transcriptional Regulation of the Human TLR9 Gene
J. Immunol.,
August 15, 2004;
173(4):
2552 - 2561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Q. Shen, M. Lu, T. Ikawa, K. Masuda, K. Ohmura, N. Minato, Y. Katsura, and H. Kawamoto
T/NK Bipotent Progenitors in the Thymus Retain the Potential to Generate Dendritic Cells
J. Immunol.,
October 1, 2003;
171(7):
3401 - 3406.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
