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Prepublished online as a Blood First Edition Paper on November 21, 2002; DOI 10.1182/blood-2002-07-2224. This Article Full Text Full Text (PDF) Supplemental Figure All Versions of this Article: 2002-07-2224v1 101/6/2167    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Verhoeyen, E. Articles by Cosset, F.-L. Search for Related Content PubMed PubMed Citation Articles by Verhoeyen, E. Articles by Cosset, F.-L. Related Collections Immunobiology Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 March 2003, Vol. 101, No. 6, pp. 2167-2174 GENE THERAPY IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes Els Verhoeyen, Valerie Dardalhon, Odile Ducrey-Rundquist, Didier Trono, Naomi Taylor, and François-Loïc Cosset From the Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, Ecole Normale Supérieure de Lyon, Lyon, France; Institut de Génétique Moléculaire de Montpellier, Montpellier, France; and Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Geneva, Switzerland. Important gene therapy target cells such as resting human T cells are refractory to transduction with lentiviral vectors. Completion of reverse transcription, nuclear import, and subsequent integration of the lentiviral genome occur in these cells only if they have been activated. In T-cell-based gene therapy trials performed to date, cells have been activated via their cognate antigen receptor. To couple activation with gene transfer, we previously generated lentiviral vectors displaying an anti-CD3 scFv fragment that allowed up to 48% transduction of freshly isolated T cells. However, transduction of highly purified resting T cells with these anti-CD3-displaying lentiviral vectors was inefficient and shifted the T cells from the naive to the memory phenotype. Here, we describe interleukin-7 (IL-7)-displaying HIV-1-derived vectors. Like recombinant IL-7, these modified particles could promote the survival of primary T cells placed in culture without inducing a naive-to-memory phenotypic switch. Furthermore, a single exposure to the IL-7-displaying vectors resulted in efficient gene transfer in both resting memory adult T cells and naive cord blood T cells. With adult naive T cells, preactivation with recombinant IL-7 was necessary for efficient gene transfer. Altogether, these results suggest that IL-7-displaying vectors could constitute interesting tools for T-cell-targeted gene therapy. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. M. Doubrovin, E. S. Doubrovina, P. Zanzonico, M. Sadelain, S. M. Larson, and R. J. O'Reilly In vivo Imaging and Quantitation of Adoptively Transferred Human Antigen-Specific T Cells Transduced to Express a Human Norepinephrine Transporter Gene Cancer Res., December 15, 2007; 67(24): 11959 - 11969. [Abstract] [Full Text] [PDF] H. J. Kueng, V. M. Leb, D. Haiderer, G. Raposo, C. Thery, S. V. Derdak, K. G. 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[Abstract] [Full Text] [PDF] S. Karlsson Gene therapy: efficient targeting of hematopoietic stem cells Blood, November 15, 2005; 106(10): 3333 - 3333. [Full Text] [PDF] E. Verhoeyen, M. Wiznerowicz, D. Olivier, B. Izac, D. Trono, A. Dubart-Kupperschmitt, and F.-L. Cosset Novel lentiviral vectors displaying "early-acting cytokines" selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cells Blood, November 15, 2005; 106(10): 3386 - 3395. [Abstract] [Full Text] [PDF] S. Cavalieri, S. Cazzaniga, M. Geuna, Z. Magnani, C. Bordignon, L. Naldini, and C. Bonini Human T lymphocytes transduced by lentiviral vectors in the absence of TCR activation maintain an intact immune competence Blood, July 15, 2003; 102(2): 497 - 505. [Abstract] [Full Text] [PDF] S. Jaleco, L. Swainson, V. Dardalhon, M. Burjanadze, S. Kinet, and N. 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