SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on November 14, 2002; DOI 10.1182/blood-2002-04-1076. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-04-1076v1 101/6/2215    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Masuya, M. Articles by Ogawa, M. Search for Related Content PubMed PubMed Citation Articles by Masuya, M. Articles by Ogawa, M. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 March 2003, Vol. 101, No. 6, pp. 2215-2218 HEMATOPOIESIS Hematopoietic origin of glomerular mesangial cells Masahiro Masuya, Christopher J. Drake, Paul A. Fleming, Christopher M. Reilly, Haiqun Zeng, William D. Hill, Angeline Martin-Studdard, David C. Hess, and Makio Ogawa From the Department of Veterans Affairs Medical Center, Department of Medicine and Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC; and the Departments of Neurology and Anatomy and Cell Biology, Medical College of Georgia and the Department of Veterans Affairs Medical Center, Augusta, GA. It was recently reported that crude bone marrow cells have the ability to differentiate into glomerular mesangial cells. However, the exact nature of the engrafting cells in the bone marrow was not known. We tested the hypothesis that hematopoietic stem cells are capable of reconstituting the mesangial cells by transplanting a clonal population of cells derived from a single stem cell. We cultured Lin, Sca-1+, c-kit+, CD34 bone marrow cells from transgenic enhanced green fluorescent protein (EGFP) mice (C57BL/6-Ly-5.2 background) individually for 1 week in the presence of interleukin-11 and steel factor. We then transplanted viable clones individually into lethally irradiated C57BL/6-Ly-5.1 mice. Kidneys from 5 recipient mice showing high levels (60%-90%) of multilineage hematopoietic reconstitution were examined 2 to 6 months later, using differential interference contrast and epifluorescence microscopy. EGFP+ cells with a morphology characteristic of mesangial cells were evident within the glomeruli. Transplantation of 100 noncultured Lin, Sca-1+, c-kit+, CD34 bone marrow cells also generated mesangial cells. Cultured EGFP+ glomerular cells from recipient mice contracted in response to angiotensin II. EGFP+ mesangial cells seen in male-to-male transplants revealed only one Y-chromosome. These data demonstrate that a single hematopoietic stem cell is capable of differentiating into glomerular mesangial cells and that the process does not involve cell fusion. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. E. Quaggin and J. A. Kreidberg Development of the renal glomerulus: good neighbors and good fences Development, February 15, 2008; 135(4): 609 - 620. [Abstract] [Full Text] [PDF] E. Miyata, M. Masuya, S. Yoshida, S. Nakamura, K. Kato, Y. Sugimoto, T. Shibasaki, K. Yamamura, K. Ohishi, K. Nishii, et al. Hematopoietic origin of hepatic stellate cells in the adult liver Blood, February 15, 2008; 111(4): 2427 - 2435. [Abstract] [Full Text] [PDF] G. Stokman, J. C. Leemans, I. Stroo, I. Hoedemaeker, N. Claessen, G. J. D. Teske, J. J. Weening, and S. Florquin Enhanced mobilization of bone marrow cells does not ameliorate renal fibrosis Nephrol. Dial. Transplant., February 1, 2008; 23(2): 483 - 491. [Abstract] [Full Text] [PDF] M. R. Vaughan and S. E. Quaggin How Do Mesangial and Endothelial Cells Form the Glomerular Tuft? J. Am. Soc. Nephrol., January 1, 2008; 19(1): 24 - 33. [Abstract] [Full Text] [PDF] J. Tongers and D. W. Losordo Frontiers in Nephrology: The Evolving Therapeutic Applications of Endothelial Progenitor Cells J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2843 - 2852. [Abstract] [Full Text] [PDF] B. Terrier, Y. Delmas, A. Hummel, C. Presne, F. Glowacki, B. Knebelmann, C. Combe, P. Lesavre, N. Maillard, L.-H. Noel, et al. Post-allogeneic haematopoietic stem cell transplantation membranous nephropathy: clinical presentation, outcome and pathogenic aspects Nephrol. Dial. Transplant., May 1, 2007; 22(5): 1369 - 1376. [Abstract] [Full Text] [PDF] M. Ogawa, A. C. LaRue, and C. J. Drake Hematopoietic origin of fibroblasts/myofibroblasts: its pathophysiologic implications Blood, November 1, 2006; 108(9): 2893 - 2896. [Abstract] [Full Text] [PDF] C. A. Eisenberg, J. B.E. Burch, and L. M. Eisenberg Bone Marrow Cells Transdifferentiate to Cardiomyocytes When Introduced into the Embryonic Heart Stem Cells, May 1, 2006; 24(5): 1236 - 1245. [Abstract] [Full Text] [PDF] R. P. Visconti, Y. Ebihara, A. C. LaRue, P. A. Fleming, T. C. McQuinn, M. Masuya, H. Minamiguchi, R. R. Markwald, M. Ogawa, and C. J. Drake An In Vivo Analysis of Hematopoietic Stem Cell Potential: Hematopoietic Origin of Cardiac Valve Interstitial Cells Circ. Res., March 17, 2006; 98(5): 690 - 696. [Abstract] [Full Text] [PDF] J. Perry, S. Tam, K. Zheng, Y. Sado, H. Dobson, B. Jefferson, R. Jacobs, and P. S. Thorner Type IV Collagen Induces Podocytic Features in Bone Marrow Stromal Stem Cells In Vitro J. Am. Soc. Nephrol., January 1, 2006; 17(1): 66 - 76. [Abstract] [Full Text] [PDF] T. Yokota, Y. Kawakami, Y. Nagai, J.-x. Ma, J.-Y. Tsai, P. W. Kincade, and S. Sato Bone Marrow Lacks a Transplantable Progenitor for Smooth Muscle Type {alpha}-Actin-Expressing Cells Stem Cells, January 1, 2006; 24(1): 13 - 22. [Abstract] [Full Text] [PDF] Z. Feng, A. R. Plati, Q.-l. Cheng, M. Berho, A. Banerjee, M. Potier, W.-c. Jy, A. Koff, L. J. Striker, and G. E. Striker Glomerular Aging in Females Is a Multi-Stage Reversible Process Mediated by Phenotypic Changes in Progenitors Am. J. Pathol., August 1, 2005; 167(2): 355 - 363. [Abstract] [Full Text] [PDF] G. Stokman, J. C. Leemans, N. Claessen, J. J. Weening, and S. Florquin Hematopoietic Stem Cell Mobilization Therapy Accelerates Recovery of Renal Function Independent of Stem Cell Contribution J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1684 - 1692. [Abstract] [Full Text] [PDF] T.-C. Fang, M. R. Alison, H. T. Cook, R. Jeffery, N. A. Wright, and R. Poulsom Proliferation of Bone Marrow-Derived Cells Contributes to Regeneration after Folic Acid-Induced Acute Tubular Injury J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1723 - 1732. [Abstract] [Full Text] [PDF] V. Schachinger and A. M. Zeiher Stem Cells and Cardiovascular and Renal Disease: Today and Tomorrow J. Am. Soc. Nephrol., March 1, 2005; 16(3_suppl_1): S2 - S6. [Abstract] [Full Text] [PDF] M. S. Szczypka, A. J. Westover, S. G. Clouthier, J. L.M. Ferrara, and H. D. Humes Rare Incorporation of Bone Marrow-Derived Cells Into Kidney After Folic Acid-Induced Injury Stem Cells, January 1, 2005; 23(1): 44 - 54. [Abstract] [Full Text] [PDF] S. Jiang, L. Walker, M. Afentoulis, D. A. Anderson, L. Jauron-Mills, C. L. Corless, and W. H. Fleming Transplanted human bone marrow contributes to vascular endothelium PNAS, November 30, 2004; 101(48): 16891 - 16896. [Abstract] [Full Text] [PDF] F. Zheng, F. Cornacchia, I. Schulman, A. Banerjee, Q.-l. Cheng, M. Potier, A. R. Plati, M. Berho, S. J. Elliot, J. Li, et al. Development of Albuminuria and Glomerular Lesions in Normoglycemic B6 Recipients of db/db Mice Bone Marrow: The Role of Mesangial Cell Progenitors Diabetes, September 1, 2004; 53(9): 2420 - 2427. [Abstract] [Full Text] [PDF] F. Togel, J. Isaac, and C. Westenfelder Hematopoietic Stem Cell Mobilization-Associated Granulocytosis Severely Worsens Acute Renal Failure J. Am. Soc. Nephrol., May 1, 2004; 15(5): 1261 - 1267. [Abstract] [Full Text] [PDF] G. Prindull and D. Zipori Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm Blood, April 15, 2004; 103(8): 2892 - 2899. [Abstract] [Full Text] [PDF] M R Alison, R Poulsom, W R Otto, P Vig, M Brittan, N C Direkze, M Lovell, T C Fang, S L Preston, and N A Wright Recipes for adult stem cell plasticity: fusion cuisine or readymade? J. Clin. Pathol., February 1, 2004; 57(2): 113 - 120. [Abstract] [Full Text] [PDF] A. Maeshima, S. Yamashita, and Y. Nojima Identification of Renal Progenitor-Like Tubular Cells that Participate in the Regeneration Processes of the Kidney J. Am. Soc. Nephrol., December 1, 2003; 14(12): 3138 - 3146. [Abstract] [Full Text] [PDF] E. L. Herzog, L. Chai, and D. S. Krause Plasticity of marrow-derived stem cells Blood, November 15, 2003; 102(10): 3483 - 3493. [Abstract] [Full Text] [PDF] M. Korbling and Z. Estrov Adult Stem Cells for Tissue Repair -- A New Therapeutic Concept? N. Engl. J. Med., August 7, 2003; 349(6): 570 - 582. [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020