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Prepublished online as a Blood First Edition Paper on December 12, 2002; DOI 10.1182/blood-2002-06-1830. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-06-1830v1 101/8/2999    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Devine, S. M. Articles by Hoffman, R. Search for Related Content PubMed PubMed Citation Articles by Devine, S. M. Articles by Hoffman, R. Related Collections Hematopoiesis and Stem Cells Transplantation Brief Reports Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 April 2003, Vol. 101, No. 8, pp. 2999-3001 HEMATOPOIESIS Brief report Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into nonhuman primates Steven M. Devine, Carrington Cobbs, Matt Jennings, Amelia Bartholomew, and Ron Hoffman From the Section of Hematology/Oncology, Department of Surgery, University of Illinois College of Medicine, Chicago; and Osiris Therapeutics, Baltimore, MD. Ex vivo-expanded mesenchymal stem cells (MSCs) were transduced with a green fluorescent protein (GFP) retroviral construct and subsequently infused into 3 adult baboons following lethal total body irradiation and hematopoietic support or without any prior conditioning. To study the long-term fate of these MSCs, necropsies were performed between 9 and 21 months following MSC infusion, and an average of 16 distinct tissues were recovered from each recipient and evaluated for the presence of the GFP transgene in purified genomic DNA by sensitive real-time polymerase chain reaction (PCR). Two baboons received autologous and one allogeneic GFP-transduced MSCs. Both allogeneic and autologous MSCs appeared to distribute in a similar manner. Gastrointestinal tissues harbored high concentrations of transgene per microgram of DNA. Additional tissues including kidney, lung, liver, thymus, and skin were also found to contain relatively high amounts of DNA equivalents. Estimated levels of engraftment in these tissues ranged from 0.1% to 2.7%. The nonconditioned recipient appeared to have less abundant engraftment. These data suggest that MSCs initially distribute broadly following systemic infusion and later may participate in ongoing cellular turnover and replacement in a wide variety of tissues. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H Aurich, M Sgodda, P Kaltwasser, M Vetter, A Weise, T Liehr, M Brulport, J G Hengstler, M M Dollinger, W E Fleig, et al. Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo Gut, April 1, 2009; 58(4): 570 - 581. [Abstract] [Full Text] [PDF] F. Wang, J. E. Dennis, A. Awadallah, L. A. Solchaga, J. Molter, Y. Kuang, N. Salem, Y. Lin, H. Tian, J. A. Kolthammer, et al. Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imaging Physiol Genomics, March 3, 2009; 37(1): 23 - 34. [Abstract] [Full Text] [PDF] P. A. 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