|
|
Prepublished online as a Blood First Edition Paper on December 27, 2002; DOI 10.1182/blood-2002-11-3335.
 Previous Article | Table of Contents | Next Article 
Blood, 1 May 2003, Vol. 101, No. 9, pp. 3514-3519
IMMUNOBIOLOGY
The PML gene is not involved in the regulation of MHC
class I expression in human cell lines
Silvia Bruno,
Fabio Ghiotto,
Franco Fais,
Marta Fagioli,
Lucilla Luzi,
Pier Giuseppe Pelicci,
Carlo Enrico Grossi, and
Ermanno Ciccone
From the Department of Experimental Medicine, Section
of Human Anatomy, Genoa University, Genoa, Italy; the
Department of Experimental Oncology, European Institute of Oncology,
Milan, Italy; the Department of Clinical and Experimental
Medicine, Section of Internal Medicine and Oncological Sciences,
Perugia University, Perugia, Italy; and the Fondazione
Italiana per la Ricerca sul Cancro, Institute for Molecular Oncology,
Milan, Italy.
The promyelocytic leukemia gene, PML,
is a growth and transformation suppressor. An additional role for
PML as a regulator of major histocompatibility complex
(MHC) class I antigen presentation has been proposed in a
murine model, which would account for evasion from host immunity of
tumors bearing malfunctioning PML, such as acute promyelocytic
leukemia. Here we investigated a possible role of
PML for the control MHC class I expression in human cells. PML function was perturbed in human cell lines either by
PML/RAR transfection or by PML- specific RNA
interference. Impairment of wild-type PML function was proved
by a microspeckled disassembly of nuclear bodies (NBs), where the
protein is normally localized, or by their complete disappearance.
However, no MHC class I down-regulation was observed in both instances.
We next constructed a PML mutant, PML mut ex3,
that is a human homolog of the murine PML mutant, truncated in exon 3, that was shown to down-regulate murine MHC class I. PML mut
ex3 transfected in human cell lines exerted a dominant-negative
effect since no PML molecules were detected in NBs but, instead, in
perinuclear and cytoplasmic larger dotlike structures.
Nevertheless, no down-regulation of MHC class I expression was evident.
Moreover, neither transfection with PML mut ex3 nor PML-specific RNA interference affected the ability of  -interferon to
up-regulate MHC class I expression. We conclude that, in human cell
lines, PML is not involved directly in the regulation of MHC class I expression.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Haupt, S. di Agostino, I. Mizrahi, O. Alsheich-Bartok, M. Voorhoeve, A. Damalas, G. Blandino, and Y. Haupt
Promyelocytic Leukemia Protein is Required for Gain of Function by Mutant p53
Cancer Res.,
June 1, 2009;
69(11):
4818 - 4826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Crowder, O. Dahle, R. E. Davis, O. S. Gabrielsen, and S. Rudikoff
PML mediates IFN-{alpha}-induced apoptosis in myeloma by regulating TRAIL induction
Blood,
February 1, 2005;
105(3):
1280 - 1287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wang, C. Shiels, P. Sasieni, P. J. Wu, S. A. Islam, P. S. Freemont, and D. Sheer
Promyelocytic leukemia nuclear bodies associate with transcriptionally active genomic regions
J. Cell Biol.,
February 16, 2004;
164(4):
515 - 526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
