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Prepublished online as a Blood First Edition Paper on December 27, 2002; DOI 10.1182/blood-2002-07-2104. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-07-2104v1 101/9/3722    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Krampera, M. Articles by Dazzi, F. Search for Related Content PubMed PubMed Citation Articles by Krampera, M. Articles by Dazzi, F. Related Collections Hematopoiesis and Stem Cells Immunobiology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 May 2003, Vol. 101, No. 9, pp. 3722-3729 TRANSPLANTATION Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide Mauro Krampera, Sarah Glennie, Julian Dyson, Diane Scott, Ruthline Laylor, Elizabeth Simpson, and Francesco Dazzi From the Department of Immunology and Transplantation Biology, Imperial College Faculty of Medicine, Hammersmith Hospital, London, United Kingdom. Mesenchymal stem cells (MSCs) have been recently shown to inhibit T-cell proliferation to polyclonal stimuli. We characterized the effect of MSCs of bone marrow origin on the T-cell response of naive and memory T cells to their cognate antigenic epitopes. The immune response to murine male transplantation antigens, HY, was selected because the peptide identity and major histocompatibility complex (MHC) restriction of the immunodominant epitopes are known. C57BL/6 female mice immunized with male cells were the source of memory T cells, whereas C6 mice transgenic for HY-specific T-cell receptor provided naive T cells. Responder cells were stimulated in vitro with male spleen cells or HY peptides in the presence or absence of MSCs. MSCs inhibited HY-specific naive and memory T cells in a dose-dependent fashion and affected cell proliferation, cytotoxicity, and the number of interferon  (IFN-)-producing HY peptide-specific T cells. However, the MSC inhibitory effect did not selectively target antigen-reactive T cells. When MSCs were added to the T-cell cultures in a Transwell system or MSCs were replaced by MSC culture supernatant, the inhibitory activity was abrogated. T-cell reactivity was also restored if MSCs were removed from the cultures. The expression of MHC molecules and the presence in culture of antigen-presenting cells (APCs) or of CD4+/CD25+ regulatory T cells were not required for MSCs to inhibit. We conclude that MSCs inhibit naive and memory T-cell responses to their cognate antigens. Overall our data suggest that MSCs physically hinder T cells from the contact with APCs in a noncognate fashion. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. E.J. Reinders, W. E. Fibbe, and T. J. Rabelink Multipotent mesenchymal stromal cell therapy in renal disease and kidney transplantation Nephrol. Dial. Transplant., October 26, 2009; (2009) gfp552v1. [Abstract] [Full Text] [PDF] S. Baglioni, M. Francalanci, R. Squecco, A. Lombardi, G. Cantini, R. Angeli, S. Gelmini, D. Guasti, S. Benvenuti, F. Annunziato, et al. 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Ang, J. C.H. Goh, J. H.P. Hui, A. Y.T. Lim, E. H. Lee, and B. H. Lim Bone Marrow-Derived Mesenchymal Stem Cells Influence Early Tendon-Healing in a Rabbit Achilles Tendon Model J. Bone Joint Surg. Am., January 1, 2007; 89(1): 74 - 81. [Abstract] [Full Text] [PDF] K. Sato, K. Ozaki, I. Oh, A. Meguro, K. Hatanaka, T. Nagai, K. Muroi, and K. Ozawa Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells Blood, January 1, 2007; 109(1): 228 - 234. [Abstract] [Full Text] [PDF] M. R. Rosen Are Stem Cells Drugs?: The Regulation of Stem Cell Research and Development Circulation, October 31, 2006; 114(18): 1992 - 2000. [Abstract] [Full Text] [PDF] J. M. van Laar and A. Tyndall Adult stem cells in the treatment of autoimmune diseases Rheumatology, October 1, 2006; 45(10): 1187 - 1193. [Abstract] [Full Text] [PDF] A. J. Nauta, G. Westerhuis, A. B. Kruisselbrink, E. G. A. Lurvink, R. Willemze, and W. E. Fibbe Donor-derived mesenchymal stem cells are immunogenic in an allogeneic host and stimulate donor graft rejection in a nonmyeloablative setting Blood, September 15, 2006; 108(6): 2114 - 2120. [Abstract] [Full Text] [PDF] A. J. Nauta, A. B. Kruisselbrink, E. Lurvink, R. Willemze, and W. E. Fibbe Mesenchymal Stem Cells Inhibit Generation and Function of Both CD34+-Derived and Monocyte-Derived Dendritic Cells J. Immunol., August 15, 2006; 177(4): 2080 - 2087. [Abstract] [Full Text] [PDF] M. Sudres, F. Norol, A. Trenado, S. Gregoire, F. Charlotte, B. Levacher, J.-J. Lataillade, P. Bourin, X. Holy, J.-P. Vernant, et al. Bone Marrow Mesenchymal Stem Cells Suppress Lymphocyte Proliferation In Vitro but Fail to Prevent Graft-versus-Host Disease in Mice. J. Immunol., June 15, 2006; 176(12): 7761 - 7767. [Abstract] [Full Text] [PDF] B. Gangadharan, E. T. Parker, L. M. Ide, H. T. Spencer, and C. B. Doering High-level expression of porcine factor VIII from genetically modified bone marrow-derived stem cells Blood, May 15, 2006; 107(10): 3859 - 3864. [Abstract] [Full Text] [PDF] K. Fukuda and S. Yuasa Stem Cells as a Source of Regenerative Cardiomyocytes Circ. Res., April 28, 2006; 98(8): 1002 - 1013. [Abstract] [Full Text] [PDF] J. Stagg, S. Pommey, N. Eliopoulos, and J. Galipeau Interferon-{gamma}-stimulated marrow stromal cells: a new type of nonhematopoietic antigen-presenting cell Blood, March 15, 2006; 107(6): 2570 - 2577. [Abstract] [Full Text] [PDF] H. Liu, D. M. Kemeny, B. C. Heng, H. W. Ouyang, A. J. Melendez, and T. Cao The immunogenicity and immunomodulatory function of osteogenic cells differentiated from mesenchymal stem cells. J. Immunol., March 1, 2006; 176(5): 2864 - 2871. [Abstract] [Full Text] [PDF] G. M. Spaggiari, A. Capobianco, S. Becchetti, M. C. Mingari, and L. Moretta Mesenchymal stem cell-natural killer cell interactions: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2-induced NK-cell proliferation Blood, February 15, 2006; 107(4): 1484 - 1490. [Abstract] [Full Text] [PDF] J. J. Minguell and A. Erices Mesenchymal Stem Cells and the Treatment of Cardiac Disease Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49. [Abstract] [Full Text] [PDF] A. Corcione, F. Benvenuto, E. Ferretti, D. Giunti, V. Cappiello, F. Cazzanti, M. Risso, F. Gualandi, G. L. Mancardi, V. Pistoia, et al. Human mesenchymal stem cells modulate B-cell functions Blood, January 1, 2006; 107(1): 367 - 372. [Abstract] [Full Text] [PDF] N. Eliopoulos, J. Stagg, L. Lejeune, S. Pommey, and J. Galipeau Allogeneic marrow stromal cells are immune rejected by MHC class I- and class II-mismatched recipient mice Blood, December 15, 2005; 106(13): 4057 - 4065. [Abstract] [Full Text] [PDF] E. Zappia, S. Casazza, E. Pedemonte, F. Benvenuto, I. Bonanni, E. Gerdoni, D. Giunti, A. Ceravolo, F. Cazzanti, F. Frassoni, et al. Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy Blood, September 1, 2005; 106(5): 1755 - 1761. [Abstract] [Full Text] [PDF] M. Krampera, A. Pasini, A. Rigo, M. T. Scupoli, C. Tecchio, G. Malpeli, A. Scarpa, F. Dazzi, G. Pizzolo, and F. Vinante HB-EGF/HER-1 signaling in bone marrow mesenchymal stem cells: inducing cell expansion and reversibly preventing multilineage differentiation Blood, July 1, 2005; 106(1): 59 - 66. [Abstract] [Full Text] [PDF] X.-X. Jiang, Y. Zhang, B. Liu, S.-X. Zhang, Y. Wu, X.-D. Yu, and N. Mao Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells Blood, May 15, 2005; 105(10): 4120 - 4126. [Abstract] [Full Text] [PDF] S. Glennie, I. Soeiro, P. J. Dyson, E. W.-F. Lam, and F. Dazzi Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells Blood, April 1, 2005; 105(7): 2821 - 2827. [Abstract] [Full Text] [PDF] P. Terness, J.-J. Chuang, T. Bauer, L. Jiga, and G. Opelz Regulation of human auto- and alloreactive T cells by indoleamine 2,3-dioxygenase (IDO)-producing dendritic cells: too much ado about IDO? Blood, March 15, 2005; 105(6): 2480 - 2486. [Abstract] [Full Text] [PDF] S. Beyth, Z. Borovsky, D. Mevorach, M. Liebergall, Z. Gazit, H. Aslan, E. Galun, and J. Rachmilewitz Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T-cell unresponsiveness Blood, March 1, 2005; 105(5): 2214 - 2219. [Abstract] [Full Text] [PDF] M. F. Pittenger and B. J. Martin Mesenchymal Stem Cells and Their Potential as Cardiac Therapeutics Circ. Res., July 9, 2004; 95(1): 9 - 20. [Abstract] [Full Text] [PDF] R. Meisel, A. Zibert, M. Laryea, U. Gobel, W. Daubener, and D. Dilloo Human bone marrow stromal cells inhibit allogeneic T-cell responses by indoleamine 2,3-dioxygenase-mediated tryptophan degradation Blood, June 15, 2004; 103(12): 4619 - 4621. [Abstract] [Full Text] [PDF] K.H. Grinnemo, A. Mansson, G. Dellgren, D. Klingberg, E. Wardell, V. Drvota, C. Tammik, J. Holgersson, O. Ringden, C. Sylven, et al. Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium J. Thorac. Cardiovasc. Surg., May 1, 2004; 127(5): 1293 - 1300. [Abstract] [Full Text] [PDF] M. B. P. Soares, R. S. Lima, L. L. Rocha, C. M. Takyia, L. Pontes-de-Carvalho, A. C. Campos de Carvalho, and R. Ribeiro-dos-Santos Transplanted Bone Marrow Cells Repair Heart Tissue and Reduce Myocarditis in Chronic Chagasic Mice Am. J. Pathol., February 1, 2004; 164(2): 441 - 447. [Abstract] [Full Text] [PDF] F. Djouad, P. Plence, C. Bony, P. Tropel, F. Apparailly, J. Sany, D. Noel, and C. Jorgensen Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals Blood, November 15, 2003; 102(10): 3837 - 3844. [Abstract] [Full Text] [PDF]

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