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Blood, 15 November 2003, Vol. 102, No. 10, pp. 3575-3583.
Prepublished online as a Blood First Edition Paper on July 31, 2003; DOI 10.1182/blood-2003-04-1154.
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HEMATOPOIESIS
Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels
Norio Suzuki,
Naruyoshi Suwabe,
Osamu Ohneda,
Naoshi Obara,
Shigehiko Imagawa,
Xiaoqing Pan,
Hozumi Motohashi, and
Masayuki Yamamoto
From the Center for Tsukuba Advanced Research Alliance (TARA), Institutes of Basic Medical Sciences, Institutes of Clinical Medicine, and Exploratory Research for Advanced Technology (ERATO) Environmental Response Project, University of Tsukuba, Japan.
Transcription factor GATA-1 is essential for the development of the erythroid lineage. To ascertain whether strict control of GATA-1 expression level is necessary for achieving proper erythropoiesis, we established transgenic mouse lines expressing green fluorescent protein (GFP) under the control of the GATA-1 gene hematopoietic regulatory domain. We examined the GATA-1 expression level by exploiting the transgenic mice and found 2 GFP-positive hematopoietic progenitor fractions in the bone marrow. One is the GFPhigh fraction containing mainly CFU-E and proerythroblasts, which coexpress transferrin receptor, while the other is the GFPlow/transferrin receptor-negative fraction containing BFU-E. Since the intensity of green fluorescence correlates well with the expression level of GATA-1, these results indicate that GATA-1 is highly expressed in erythroid colony-forming unit (CFU-E) but low in erythroid burst-forming unit (BFU-E), suggesting that the incremental expression of GATA-1 is required for the formation of erythroid progenitors. We also examined GFP-positive fractions in the transgenic mouse spleen and fetal liver and identified fractions containing BFU-E and CFU-E, respectively. This study also presents an efficient method for enriching the CFU-E and BFU-E from mouse hematopoietic tissues. (Blood. 2003;102:3575-3583)
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