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Blood, 15 December 2003, Vol. 102, No. 13, pp. 4329-4335. Prepublished online as a Blood First Edition Paper on June 19, 2003; DOI 10.1182/blood-2003-01-0082. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-01-0082v1 102/13/4329    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Horn, P. A. Articles by Kiem, H.-P. Search for Related Content PubMed PubMed Citation Articles by Horn, P. A. Articles by Kiem, H.-P. Related Collections Hematopoiesis and Stem Cells Transplantation Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates Peter A. Horn, Bobbie M. Thomasson, Brent L. Wood, Robert G. Andrews, Julia C. Morris, and Hans-Peter Kiem From the Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA; and the Departments of Laboratory Medicine, Pediatrics, and Medicine, University of Washington School of Medicine, Seattle. The nonobese diabetic/severe combined immune-deficient (NOD/SCID) mouse xenotransplantation assay is the most commonly used surrogate assay for the study of human candidate stem cells. In contrast to large animal and human studies, however, it is limited by the short life span of the recipients, the limited proliferative demand placed on the transplanted cells, and the inability to support differentiation into all hematopoietic lineages. In the present study, we directly compared hematopoietic repopulation in NOD/SCID mice with autologous reconstitution in the baboon, a well-established preclinical large animal model for stem cell transplantation. Baboon CD34-enriched marrow cells were retrovirally marked and infused into the irradiated baboon and the NOD/SCID mice. Although the percentage of gene-marked cells was high and remained stable in NOD/SCID mice up to 12 weeks and in those that underwent secondary transplantation, we observed a considerable decline and overall a significantly (10-fold) lower percentage of gene-marked cells in the baboons. In addition, clonal integration site analysis revealed common proviral vector integrants in NOD/SCID repopulating cells and in the baboon at 6 weeks but not at 6 months after transplantation. These results suggest that distinct hematopoietic stem/progenitor cells are responsible for hematopoietic reconstitution in NOD/SCID mice compared with nonhuman primates. (Blood. 2003;102:4329-4335) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. S. Knudsen, A. N. Allen, D. F. McLerran, R. L. Vessella, J. Karademos, J. E. Davies, B. Maqsodi, G. K. McMaster, and A. R. Kristal Evaluation of the Branched-Chain DNA Assay for Measurement of RNA in Formalin-Fixed Tissues J. Mol. Diagn., March 1, 2008; 10(2): 169 - 176. [Abstract] [Full Text] [PDF] P. A. Horn, H.-P. Kiem, K. Ando, and T. Yahata Expansion of SCID repopulating cells does not prove expansion of hematopoietic stem cells. Blood, July 15, 2006; 108(2): 771 - 772. [Full Text] [PDF] C. Delaney, B. Varnum-Finney, K. Aoyama, C. Brashem-Stein, and I. D. Bernstein Dose-dependent effects of the Notch ligand Delta1 on ex vivo differentiation and in vivo marrow repopulating ability of cord blood cells Blood, October 15, 2005; 106(8): 2693 - 2699. [Abstract] [Full Text] [PDF] H. Hanawa, P. Hematti, K. Keyvanfar, M. E. Metzger, A. Krouse, R. E. Donahue, S. Kepes, J. Gray, C. E. Dunbar, D. A. Persons, et al. Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus-based lentiviral vector system Blood, June 1, 2004; 103(11): 4062 - 4069. [Abstract] [Full Text] [PDF] D.-W. Kim, Y.-J. Chung, T.-G. Kim, Y.-L. Kim, and I.-H. Oh Cotransplantation of third-party mesenchymal stromal cells can alleviate single-donor predominance and increase engraftment from double cord transplantation Blood, March 1, 2004; 103(5): 1941 - 1948. [Abstract] [Full Text] [PDF]

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