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Prepublished online as a Blood First Edition Paper on March 20, 2003; DOI 10.1182/blood-2002-08-2437. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-08-2437v1 102/2/705    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Omodeo-Salè, F. Articles by Taramelli, D. Search for Related Content PubMed PubMed Citation Articles by Omodeo-Salè, F. Articles by Taramelli, D. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 July 2003, Vol. 102, No. 2, pp. 705-711 RED CELLS Accelerated senescence of human erythrocytes cultured with Plasmodium falciparum Fausta Omodeo-Salè, Anna Motti, Nicoletta Basilico, Silvia Parapini, Piero Olliaro, and Donatella Taramelli From the Istituto di Fisiologia Generale e Chimica Biologica Giovanni Esposito, Milan, Italy; Istituto di Microbiologia, Università di Milano, Milan, Italy; United Nations Development Programme/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases (TDR), Geneva, Switzerland Red blood cells infected withPlasmodium falciparum(IRBCs) undergo changes primarily in their membrane composition that contribute to malaria pathogenesis. However, all manifestations (eg, anemia) cannot be accounted for by IRBCs alone. Uninfected erythrocytes (URBCs) may play a role, but they have been under-researched. We wanted to document changes in the erythrocyte membrane that could contribute to URBC reduced life span and malaria-associated anemia. Human erythrocytes were cultured withP falciparumand washed at the trophozoite stage. IRBCs and URBCs were separated on Percoll density gradient, thus obtaining erythrocyte fractions of different densities/ages. IRBC- and URBC-purified membranes were analyzed and compared with control normal erythrocytes (NRBCs) of the same age, from the same donor, kept in the same conditions.P falciparumaccelerated aging of both IRBCs and URBCs, causing a significant shift in the cell population toward the denser (old) fraction. Protein, phospholipid, and cholesterol content were reduced in IRBCs and young URBCs. Young and medium uninfected fractions had higher levels of lipid peroxidation and phospholipid saturation (because of the loss of polyunsaturated fatty acids, PUFAs) and lower phosphatidylserine. In IRBCs, thiobarbituric reactive substances (TBARSs) were higher, and PUFAs and phosphatidylserine lower than in NRBCs and URBCs. In comparison, trophozoite membranes had lower phospholipid (particularly sphingomyelin and phosphatidylserine) and cholesterol content and a higher degree of saturation. Parasite-induced peroxidative damage might account for these modifications. In summary, we demonstrated that membrane damage leading to accelerated senescence of both infected and uninfected erythrocytes will likely contribute to malaria anemia. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Nuchsongsin, K. Chotivanich, P. Charunwatthana, O.-S. Fausta, D. Taramelli, N. P. Day, N. J. White, and A. M. Dondorp Effects of Malaria Heme Products on Red Blood Cell Deformability Am J Trop Med Hyg, October 1, 2007; 77(4): 617 - 622. [Abstract] [Full Text] [PDF] P. G. McQueen and F. E. McKenzie Age-structured red blood cell susceptibility and the dynamics of malaria infections PNAS, June 15, 2004; 101(24): 9161 - 9166. [Abstract] [Full Text] [PDF]

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