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Prepublished online as a Blood First Edition Paper on June 5, 2003; DOI 10.1182/blood-2003-01-0180. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-01-0180v1 102/7/2459    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lieby, P. Articles by Martin, T. Search for Related Content PubMed PubMed Citation Articles by Lieby, P. Articles by Martin, T. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 October 2003, Vol. 102, No. 7, pp. 2459-2465 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Memory B cells producing somatically mutated antiphospholipid antibodies are present in healthy individuals Patricia Lieby, Anne Soley, Anne-Marie Knapp, Martine Cerutti, Jean-Marie Freyssinet, Jean-Louis Pasquali, and Thierry Martin From the Laboratoire d'Immunopathologie, Institut National de la Santé etdela Recherche Médicale (INSERM) EMI 0222, Institut d'Hématologie et d'Immunologie, Hôpital Civil, Faculté deMédecine de Strasbourg, Strasbourg, France; Laboratoire de Pathologie Comparée, Institut National pour la Recherche Agronomique/Institut National pour la Recherche Scientifique (INRA/CNRS) URA2209, Saint Christol lez-Alès, France; Laboratoire d'Hématologie, Institut d'Hématologie et d'Immunologie, Hôpital Civil, Faculté deMédecine de Strasbourg, Strasbourg, France; and the Unité 143 INSERM, Hôpital de Bicêtre, Le Kremlin Bicêtre, France. Antiphospholipid antibodies (aPLs) are associated with thrombosis and recurrent abortions during autoimmune pathologies, but they are also produced in healthy individuals and during infectious diseases. To analyze the possible links between physiologic and pathologic aPLs, it is of importance to characterize normal aPL production. We took advantage of the known tropism of Epstein-Barr virus (EBV) for B cells in general, and memory B cells in particular, during primary infectious mononucleosis (IMN) in 3 patients to get access to anticardiolipin (aCL)-producing B cells. Flow cytometry analysis of these cells showed that, depending on the patient, 10% to 60% of immunoglobulin M (IgM) aCL-producing B cells express the CD27 marker of memory B cells. Single cell sorting of aCL B cells, followed by single cell reverse transcription-polymerase chain reaction (RT-PCR) amplification of their immunoglobulin variable region genes, showed that some of these cells produce mutated forms of aCL antibodies, confirming their memory B-cell origin. Considering that, during primary IMN, EBV infects and expands already pre-existing memory B cells, we conclude that healthy individuals have a discrete pool of aCL memory cells able to produce mutated forms of antibodies. The implications of this new information are discussed in light of different hypotheses regarding the origin of aCL. (Blood. 2003;102:2459-2465) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. C. Chu, R. Catera, K. Hatzi, X.-J. Yan, L. Zhang, X. B. Wang, H. M. Fales, S. L. Allen, J. E. Kolitz, K. R. Rai, et al. Chronic lymphocytic leukemia antibodies with a common stereotypic rearrangement recognize nonmuscle myosin heavy chain IIA Blood, December 15, 2008; 112(13): 5122 - 5129. [Abstract] [Full Text] [PDF] K. Stamatopoulos, C. Belessi, C. Moreno, M. Boudjograh, G. Guida, T. Smilevska, L. Belhoul, S. Stella, N. Stavroyianni, M. Crespo, et al. Over 20% of patients with chronic lymphocytic leukemia carry stereotyped receptors: pathogenetic implications and clinical correlations Blood, January 1, 2007; 109(1): 259 - 270. [Abstract] [Full Text] [PDF] P. Lieby, V. Poindron, S. Roussi, C. Klein, A.-M. Knapp, J.-C. Garaud, M. Cerutti, T. Martin, and J.-L. Pasquali Pathogenic antiphospholipid antibody: an antigen-selected needle in a haystack Blood, September 15, 2004; 104(6): 1711 - 1715. [Abstract] [Full Text] [PDF]

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