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Prepublished online as a Blood First Edition Paper on July 10, 2003; DOI 10.1182/blood-2002-12-3929. This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 2002-12-3929v1 102/7/2547    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Okumura, S. Articles by Okayama, Y. Search for Related Content PubMed PubMed Citation Articles by Okumura, S. Articles by Okayama, Y. Related Collections Immunobiology Phagocytes Gene Expression Genomics Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 October 2003, Vol. 102, No. 7, pp. 2547-2554 IMMUNOBIOLOGY Identification of specific gene expression profiles in human mast cells mediated by Toll-like receptor 4 and FcRI Shigeru Okumura, Jun-ichi Kashiwakura, Hisashi Tomita, Kenji Matsumoto, Toshiharu Nakajima, Hirohisa Saito, and Yoshimichi Okayama From the Laboratory of Allergy Transcriptome, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Yokohama, Japan; Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; and Department of Microbiology, Tokyo Dental College, Chiba, Japan. Rodent mast cells (MCs) are reported to play a pivotal role in both innate and adaptive immunity. However, there is so far no evidence that human MCs are involved in innate immunity. We found that a functional Toll-like receptor 4 (TLR4) was expressed on human MCs when it was up-regulated by interferon (IFN-). To systematically explore how human MCs modulate the immune system following TLR4-mediated activation and FcRI aggregation, we used high-density oligonucleotide probe arrays (GeneChip) to compare the lipopolysaccharide (LPS)-induced gene expression profile with the IgE/anti-IgE-mediated profile in MCs. Both a shared core response, and LPS- or anti-IgE-specific programs of gene expression were observed in MCs. Furthermore, MCs exhibited an antiviral response gene program in response to IFN-, and LPS sustained that expression. Compared with the LPS-stimulated gene expression profile of human peripheral blood mononuclear cells, LPS-stimulated MCs specifically induced a subset of genes that included a Th2 cytokine and chemokines that recruit Th2 cells and eosinophils. These results reveal that human MCs express tailored pathogen- and antigen-specific immune responses and that human MCs may play important roles in innate and adaptive immunity.(Blood. 2003;102:2547-2554) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Okayama, S. Okumura, H. Sagara, K. Yuki, T. Sasaki, N. Watanabe, M. Fueki, K. Sugiyama, K. Takeda, T. Fukuda, et al. Fc{epsilon}RI-mediated thymic stromal lymphopoietin production by interleukin-4-primed human mast cells Eur. Respir. J., August 1, 2009; 34(2): 425 - 435. [Abstract] [Full Text] [PDF] P. Bradding Human lung mast cell heterogeneity Thorax, April 1, 2009; 64(4): 278 - 280. [Full Text] [PDF] S. R. Holdsworth and S. A. Summers Role of Mast Cells in Progressive Renal Diseases J. Am. Soc. Nephrol., December 1, 2008; 19(12): 2254 - 2261. [Abstract] [Full Text] [PDF] Y. Fukuoka, H.-Z. Xia, L. B. Sanchez-Munoz, A. L. Dellinger, L. 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