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Blood, 15 October 2003, Vol. 102, No. 8, pp. 2851-2855. Prepublished online as a Blood First Edition Paper on June 19, 2003; DOI 10.1182/blood-2003-04-1224. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-04-1224v1 102/8/2851    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yang, T. L. Articles by Ginsburg, D. Search for Related Content PubMed PubMed Citation Articles by Yang, T. L. Articles by Ginsburg, D. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Biosynthetic origin and functional significance of murine platelet factor V Tony L. Yang, Steven W. Pipe, Angela Yang, and David Ginsburg From the Department of Human Genetics, Department of Pediatrics, and Howard Hughes Medical Institute, University of Michigan; and the Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan, Ann Arbor, MI. Factor V (FV), a central regulatory protein in hemostasis, is distributed into distinct plasma and platelet compartments. Although platelet FV is highly concentrated within the platelet -granule, previous analysis of human bone marrow and liver transplant recipients has demonstrated that platelet FV in these individuals originates entirely from the uptake of plasma FV. In order to examine further the biosynthetic origins of the platelet and plasma FV pools, we performed bone marrow transplantations of Fv-null (Fv–/–) fetal liver cells (FLCs) into wild-type mice. Fractionation of whole blood from control mice demonstrated that approximately 14% of total blood FV activity is platelet-associated. Mice that received transplants of Fv-null FLCs displayed a high degree of engraftment and appeared grossly normal, with no evidence for spontaneous hemorrhage. Although total FV levels in Fv-null FLC recipients were only mildly decreased, the FV activity within the platelet compartment was reduced to less than 1% of that in normal mice. We conclude that the murine platelet FV compartment is derived exclusively from primary biosynthesis within cells of marrow origin, presumably megakaryocytes, and that an intact platelet FV pool is not required for protection from spontaneous hemorrhage or bleeding following minor trauma. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Neyman, J. Gewirtz, and M. Poncz Analysis of the spatial and temporal characteristics of platelet-delivered factor VIII-based clots Blood, August 15, 2008; 112(4): 1101 - 1108. [Abstract] [Full Text] [PDF] B. Zhang, M. Spreafico, C. Zheng, A. Yang, P. Platzer, M. U. Callaghan, Z. Avci, N. Ozbek, J. Mahlangu, T. Haw, et al. Genotype-phenotype correlation in combined deficiency of factor V and factor VIII Blood, June 15, 2008; 111(12): 5592 - 5600. [Abstract] [Full Text] [PDF] D. T. Eitzman, R. J. Westrick, Y. Shen, P. F. Bodary, S. Gu, S. L. Manning, S. L. Dobies, and D. Ginsburg Homozygosity for Factor V Leiden Leads to Enhanced Thrombosis and Atherosclerosis in Mice Circulation, April 12, 2005; 111(14): 1822 - 1825. [Abstract] [Full Text] [PDF] S. M. Day, J. L. Reeve, B. Pedersen, D. M Farris, D. D. Myers, M. Im, T. W. Wakefield, N. Mackman, and W. P. Fay Macrovascular thrombosis is driven by tissue factor derived primarily from the blood vessel wall Blood, January 1, 2005; 105(1): 192 - 198. [Abstract] [Full Text] [PDF] H. Sun, T. L. Yang, A. Yang, X. Wang, and D. Ginsburg The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis Blood, October 15, 2003; 102(8): 2856 - 2861. [Abstract] [Full Text] [PDF]

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