SEARCH:   Advanced

Blood, 15 June 2004, Vol. 103, No. 12, pp. 4511-4513. Prepublished online as a Blood First Edition Paper on February 26, 2004; DOI 10.1182/blood-2003-07-2397. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-07-2397v1 103/12/4511    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schomber, T. Articles by Skoda, R. C. Search for Related Content PubMed PubMed Citation Articles by Schomber, T. Articles by Skoda, R. C. Related Collections Hematopoiesis and Stem Cells Gene Expression Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Brief report Gene silencing by lentivirus-mediated delivery of siRNA in human CD34+ cells Tibor Schomber, Christian P. Kalberer, Aleksandra Wodnar-Filipowicz, and Radek C. Skoda From the Department of Research, Experimental Hematology, Basel University Hospital, Basel, Switzerland. To derive an efficient system for gene silencing in human hematopoietic stem cells (HSCs) we modified a lentiviral vector for small interfering RNA (siRNA) delivery. For this purpose, an H1 promoter-driven siRNA expression cassette was introduced into a lentiviral vector, and the p53 mRNA was chosen as a target for siRNA-mediated gene silencing. Using the recombinant lentivirus we infected human cord blood-derived CD34+ cells and obtained a transfection efficiency of up to 50%, as determined by expression of enhanced green fluorescent protein (EGFP). In EGFP-positive long-term culture-initiating cell (LTC-IC)- and colony-forming unit cell (CFU-C)-derived cells, we observed a reduction of p53 mRNA of up to 95%. Importantly, this reduction remained stable during several weeks of cell culture. Furthermore, p53 gene silencing resulted in decreased p21 mRNA levels and reduced the sensitivity of CD34+ cells toward the cytotoxic drug etoposide. Thus, lentiviral delivery of siRNA can allow for efficient and stable gene silencing in human HSCs and will be very valuable for further gene function studies. (Blood. 2004;103:4511-4513) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Sun, R.-F. Guo, H. Gao, J. V. Sarma, F. S. Zetoune, and P. A. Ward Attenuation of IgG immune complex-induced acute lung injury by silencing C5aR in lung epithelial cells FASEB J, November 1, 2009; 23(11): 3808 - 3818. [Abstract] [Full Text] [PDF] R. D. Dinnen, L. Drew, D. P. Petrylak, Y. Mao, N. Cassai, J. Szmulewicz, P. Brandt-Rauf, and R. L. Fine Activation of Targeted Necrosis by a p53 Peptide: A NOVEL DEATH PATHWAY THAT CIRCUMVENTS APOPTOTIC RESISTANCE J. Biol. Chem., September 14, 2007; 282(37): 26675 - 26686. [Abstract] [Full Text] [PDF] D. De Paula, M. V. L.B. Bentley, and R. I. Mahato Hydrophobization and bioconjugation for enhanced siRNA delivery and targeting RNA, April 1, 2007; 13(4): 431 - 456. [Abstract] [Full Text] [PDF] I. Bot, J. Guo, M. Van Eck, P. J. Van Santbrink, P. H. E. Groot, R. B. Hildebrand, J. Seppen, T. J. C. Van Berkel, and E. A. L. Biessen Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo Blood, August 15, 2005; 106(4): 1147 - 1153. [Abstract] [Full Text] [PDF] G. Dotti, B. Savoldo, M. Pule, K. C. Straathof, E. Biagi, E. Yvon, S. Vigouroux, M. K. Brenner, and C. M. Rooney Human cytotoxic T lymphocytes with reduced sensitivity to Fas-induced apoptosis Blood, June 15, 2005; 105(12): 4677 - 4684. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020