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 Blood, 15 January 2004, Vol. 103, No. 2, pp. 580-582.
Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-07-2298.

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HEMATOPOIESIS
Brief report

Highly efficient lentiviral-mediated human cytokine transgenesis on the NOD/scid background

Isabel Punzon, Luis M. Criado, Alfredo Serrano, Fernando Serrano, and Antonio Bernad

From the Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Campus de Cantoblanco, Madrid, Spain.

Human neo-organ formation from stem cells can only be assayed by in vivo xenotransplantation. The human nonobese diabetic–severe combined immunodeficient (HuNOD/scid) CD34+ cell transplantation is a model that allows examination of hematopoietic tissue formation, although human hematopoietic cell maturation is abortive. Conventional humanization of the cytokine microenvironment has depended on generation of human cytokine-transgenic mice in strains appropriate for conventional plasmid microinjection, followed by backcrossing, a costly and time-consuming approach. Lentiviral vector infection of single-cell embryos was recently reported to produce transgenic animals. Using this approach, we have generated direct human granulocyte-macrophage colony-stimulating factor (hGM-CSF) transgenic mice from lentivirus-microinjected NOD/scid embryos, with 68% efficiency and 100% penetrance; this allowed us to obtain NOD/scid transgenic mice with considerable savings of resources. This powerful technique should assist in producing novel mouse models for the study of human blood cell lineage development and other human neo-organs from stem cell xenotransplantation for which a similar "humanization" rationale may be required.


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This article has been cited by other articles:


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 Physiol. GenomicsHome page
F. Park
Lentiviral vectors: are they the future of animal transgenesis?
Physiol Genomics, October 19, 2007; 31(2): 159 - 173.
[Abstract] [Full Text] [PDF]


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