SEARCH:   Advanced

Blood, 1 February 2004, Vol. 103, No. 3, pp. 1166-1170. Prepublished online as a Blood First Edition Paper on September 25, 2003; DOI 10.1182/blood-2003-06-1815. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-06-1815v1 103/3/1166    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rollini, P. Articles by Leyvraz, S. Search for Related Content PubMed PubMed Citation Articles by Rollini, P. Articles by Leyvraz, S. Related Collections Hematopoiesis and Stem Cells Transplantation Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Brief report Long-term expansion of transplantable human fetal liver hematopoietic stem cells Pierre Rollini, Stefan Kaiser, Eveline Faes-van't Hull, Ursula Kapp, and Serge Leyvraz From the Centre Pluridisciplinaire d'Oncologie, University Hospital, Lausanne, Switzerland; and the Hematology/Oncology Department, University Medical Center, Freiburg, Germany. Hematopoietic stem cells (HSCs), with their dual ability for self-renewal and multilineage differentiation, constitute an essential component of hematopoietic transplantations. Human fetal liver (FL) represents a promising alternative HSC source, and we previously reported simple culture conditions allowing long-term expansion of FL hematopoietic progenitors. In the present study, we used the nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse xenotransplantation assay to confirm that human FL is rich in NOD/SCID-repopulating cells (SRCs) and to show that these culture conditions repeatedly maintained short- and long-term SRCs from various FL samples for at least 28 days. Quantitative limited dilution analysis in NOD/SCID mice demonstrated for the first time that a 10- to over a 100-fold net expansion of FL SRCs could be achieved after 28 days of culture. The efficiency of this culture system may lead to an increase in the use of FL as a source of HSCs for transplantation in adult patients, as previously demonstrated with umbilical cord blood under different culture conditions. (Blood. 2004;103:1166-1170) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Mimeault and S. K. Batra Concise Review: Recent Advances on the Significance of Stem Cells in Tissue Regeneration and Cancer Therapies Stem Cells, November 1, 2006; 24(11): 2319 - 2345. [Abstract] [Full Text] [PDF] N. M. Masson, I. S. Currie, J. D. Terrace, O. J. Garden, R. W. Parks, and J. A. Ross Hepatic progenitor cells in human fetal liver express the oval cell marker Thy-1. Am J Physiol Gastrointest Liver Physiol, July 1, 2006; 291(1): G45 - G54. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020