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Blood, 1 March 2004, Vol. 103, No. 5, pp. 1647-1652. Prepublished online as a Blood First Edition Paper on November 26, 2003; DOI 10.1182/blood-2003-06-1967. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-06-1967v1 2003-06-1967v2 103/5/1647    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pochampally, R. R. Articles by Prockop, D. J. Search for Related Content PubMed PubMed Citation Articles by Pochampally, R. R. Articles by Prockop, D. J. Related Collections Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  GENE THERAPY Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes Radhika R. Pochampally, Jason R. Smith, Joni Ylostalo, and Darwin J. Prockop From the Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA. Recently there has been interest in developing cell and gene therapies with adult stem cells from human bone marrow referred to as mesenchymal stem cells or marrow stromal cells (hMSCs). We incubated early-passage hMSCs in serum-free medium without cytokines or other supplements for 2 to 4 weeks. Surprisingly, a subpopulation of the cells survived serum deprivation and then began to proliferate in serum-containing medium. The cells selected by serum deprivation had longer telomeres than control cells. Also, the patterns of gene expression revealed by reverse transcriptase–polymerase chain reaction (RT-PCR) assays and microarray data indicated that the cells selected by serum deprivation were a subpopulation of very early progenitor cells with enhanced expression of octomer-binding transcription factor 4 (OCT-4) and several other genes characteristically expressed in embryonic cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Z. Ratajczak, D.-M. Shin, and M. Kucia Very Small Embryonic/Epiblast-Like Stem Cells: A Missing Link To Support the Germ Line Hypothesis of Cancer Development? Am. J. Pathol., June 1, 2009; 174(6): 1985 - 1992. [Abstract] [Full Text] [PDF] F. Ng, S. Boucher, S. Koh, K. S. R. Sastry, L. Chase, U. Lakshmipathy, C. Choong, Z. Yang, M. C. Vemuri, M. S. Rao, et al. PDGF, TGF-{beta}, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages Blood, July 15, 2008; 112(2): 295 - 307. [Abstract] [Full Text] [PDF] D Mrugala, C Bony, N Neves, L Caillot, S Fabre, D Moukoko, C Jorgensen, and D Noel Phenotypic and functional characterisation of ovine mesenchymal stem cells: application to a cartilage defect model Ann Rheum Dis, March 1, 2008; 67(3): 288 - 295. [Abstract] [Full Text] [PDF] C. Jomary and S. E. Jones Induction of Functional Photoreceptor Phenotype by Exogenous Crx Expression in Mouse Retinal Stem Cells Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 429 - 437. [Abstract] [Full Text] [PDF] T. Rubinek, V. Chesnokova, I. Wolf, K. Wawrowsky, G. Vlotides, and S. Melmed Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1082 - C1092. [Abstract] [Full Text] [PDF] T.-Y. Ling, M.-D. Kuo, C.-L. Li, A. L. Yu, Y.-H. Huang, T.-J. Wu, Y.-C. Lin, S.-H. Chen, and J. Yu Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro PNAS, June 20, 2006; 103(25): 9530 - 9535. [Abstract] [Full Text] [PDF] M.-S. Tsai, S.-M. Hwang, Y.-L. Tsai, F.-C. Cheng, J.-L. Lee, and Y.-J. Chang Clonal Amniotic Fluid-Derived Stem Cells Express Characteristics of Both Mesenchymal and Neural Stem Cells Biol Reprod, March 1, 2006; 74(3): 545 - 551. [Abstract] [Full Text] [PDF] A. C. Boquest, A. Shahdadfar, K. Fronsdal, O. Sigurjonsson, S. H. Tunheim, P. Collas, and J. E. Brinchmann Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem Cells: Alteration of Gene Expression after In Vitro Cell Culture Mol. Biol. Cell, March 1, 2005; 16(3): 1131 - 1141. [Abstract] [Full Text] [PDF] D. Pain, G.-W. Chirn, C. Strassel, and D. M. Kemp Multiple Retropseudogenes from Pluripotent Cell-specific Gene Expression Indicates a Potential Signature for Novel Gene Identification J. Biol. Chem., February 25, 2005; 280(8): 6265 - 6268. [Abstract] [Full Text] [PDF] A. Hermann, R. Gastl, S. Liebau, M. O. Popa, J. Fiedler, B. O. Boehm, M. Maisel, H. Lerche, J. Schwarz, R. Brenner, et al. Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells J. Cell Sci., September 1, 2004; 117(19): 4411 - 4422. [Abstract] [Full Text] [PDF]

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