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Blood, 15 March 2004, Vol. 103, No. 6, pp. 2143-2149. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-06-2186. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-06-2186v1 103/6/2143    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bezerra, L. M. L. Articles by Filler, S. G. Search for Related Content PubMed PubMed Citation Articles by Bezerra, L. M. L. Articles by Filler, S. G. Related Collections Hemostasis, Thrombosis, and Vascular Biology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Interactions of Aspergillus fumigatus with endothelial cells: internalization, injury, and stimulation of tissue factor activity Leila M. Lopes Bezerra, and Scott G. Filler From St John's Cardiovascular Research Center, Division of Infectious Diseases, Department of Internal Medicine, Harbor-UCLA, Research and Education Institute, Torrance, California; and the Department of Cell Biology and Genetics, Biology Institute, State University of Rio de Janeiro (UERJ), Rio de Janeiro, Brazil. Invasive aspergillosis causes significant mortality among patients with hematologic malignancies. This infection is characterized by vascular invasion and thrombosis. To study the pathogenesis of invasive aspergillosis, we investigated the interactions of Aspergillus fumigatus conidia and hyphae with endothelial cells in vitro. We found that both forms of the organism induced endothelial cell microfilament rearrangement and subsequent endocytosis. Conidia were endocytosed 2-fold more avidly than hyphae, and endocytosis was independent of fungal viability. Endocytosed conidia and hyphae caused progressive endothelial cell injury after 4 hours of infection. Live conidia induced more endothelial cell injury than did live hyphae. However, endothelial cell injury caused by conidia was dependent on fungal viability, whereas injury caused by hyphae was not, indicating that conidia and hyphae injure endothelial cells by different mechanisms. Neither live nor killed conidia increased tissue factor activity of endothelial cells. In contrast, both live and killed hyphae stimulated significant endothelial cell tissue factor activity, as well as the expression of tissue factor antigen on the endothelial cell surface. These results suggest that angioinvasion and thrombosis caused by A fumigatus hyphae in vivo may be due in part to endothelial cell invasion, induction of injury, and stimulation of tissue factor activity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Y. Chiang, D. C. Sheppard, F. N. Gravelat, T. F. Patterson, and S. G. Filler Aspergillus fumigatus Stimulates Leukocyte Adhesion Molecules and Cytokine Production by Endothelial Cells In Vitro and during Invasive Pulmonary Disease Infect. Immun., August 1, 2008; 76(8): 3429 - 3438. [Abstract] [Full Text] [PDF] T. J. Kurtti and N. O. Keyhani Intracellular infection of tick cell lines by the entomopathogenic fungus Metarhizium anisopliae Microbiology, June 1, 2008; 154(6): 1700 - 1709. [Abstract] [Full Text] [PDF] C. C. Figueiredo, P. M. S. Deccache, L. M. Lopes-Bezerra, and V. Morandi TGF-beta1 induces transendothelial migration of the pathogenic fungus Sporothrix schenckii by a paracellular route involving extracellular matrix proteins Microbiology, September 1, 2007; 153(9): 2910 - 2921. [Abstract] [Full Text] [PDF] Q. T. Phan, R. A. Fratti, N. V. Prasadarao, J. E. Edwards Jr., and S. G. Filler N-cadherin Mediates Endocytosis of Candida albicans by Endothelial Cells J. Biol. Chem., March 18, 2005; 280(11): 10455 - 10461. [Abstract] [Full Text] [PDF] A. S. Ibrahim, B. Spellberg, V. Avanessian, Y. Fu, and J. E. Edwards Jr. Rhizopus oryzae Adheres to, Is Phagocytosed by, and Damages Endothelial Cells In Vitro Infect. Immun., February 1, 2005; 73(2): 778 - 783. [Abstract] [Full Text] [PDF]

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