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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2541-2546. Prepublished online as a Blood First Edition Paper on November 26, 2003; DOI 10.1182/blood-2003-09-3281. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-09-3281v1 103/7/2541    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pearce, D. J. Articles by Bonnet, D. Search for Related Content PubMed PubMed Citation Articles by Pearce, D. J. Articles by Bonnet, D. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Multiparameter analysis of murine bone marrow side population cells Daniel J. Pearce, Christopher M. Ridler, Catherine Simpson, and Dominique Bonnet From the Hematopoietic Stem Cell Laboratory and the FACS Laboratory, the London Research Institute, Cancer Research UK, London, United Kingdom. We describe the multiparameter flow cytometric analysis of the relationship between side population (SP) formation and well-characterized, antigen-defined stem cell subsets. We also compared the competitive repopulation ability of subsets defined by the SP profile to those identified by antigenic markers. The vast majority of SP cells possessed a primitive cell phenotype (c-kit+, SCA-1+, Thy-1+, CD31+, CD135neg, lineageneg), but only a minority of antigen-defined subsets were SP cells. Hence, although SP cells are identified independently of antigenic markers, they are not distinct from established stem cell phenotypes, but are a small subset of them. Approximately half of SP cells expressed CD34 at readily detectable levels, and one-third of SP cells possessed the primitive c-kit+, SCA-1+, lineageneg, CD34neg cell phenotype. Since most SP cells are a subset of c-kit+, Thy-1+, lineageneg, SCA-1+ cells (KTLS), we determined whether the SP cell subset represents a further enrichment in long-term repopulating cell content. The SP+ subset of KTLS+ cells was more enriched for competitive repopulation units than the SP- fraction of KTLS+ cells. Hence, the SP cell fraction highlights a subset of the long-term repopulating cells found within the already highly purified KTLS fraction. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Cesar, A. P. R. Abud, C. C. de Oliveira, F. Cardoso, R. P. 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