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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2577-2584. Prepublished online as a Blood First Edition Paper on November 20, 2003; DOI 10.1182/blood-2003-08-2770. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-08-2770v1 103/7/2577    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bompais, H. Articles by Uzan, G. Search for Related Content PubMed PubMed Citation Articles by Bompais, H. Articles by Uzan, G. Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Apoptosis Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Human endothelial cells derived from circulating progenitors display specific functional properties compared with mature vessel wall endothelial cells Heidi Bompais, Jalila Chagraoui, Xavier Canron, Mihaela Crisan, Xu Hui Liu, Aurora Anjo, Carine Tolla-Le Port, Marylene Leboeuf, Pierre Charbord, Andreas Bikfalvi, and Georges Uzan From INSERM U506, Hôpital Paul Brousse, Villejuif, France; INSERM EPI 0113 Bordeaux, France; Institut du Cancer et d'Immunogénétique, Hôpital Paul Brousse, Villejuif, France; and Laboratoire d'Hématopoïèse, Faculté de Médecine, Tours, France. Endothelial progenitor cells (EPCs) were shown to be present in systemic circulation and cord blood. We investigated whether EPCs display specific properties compared with mature endothelial cells. Human cord blood CD34+ cells were isolated and adherent cells were amplified under endothelial conditions. Expression of specific markers identified them as endothelial cells, also called endothelial progenitor-derived cells (EPDCs). When compared to mature endothelial cells, human umbilical vein endothelial cells (HUVECs) and human bone marrow endothelial cells (HBMECs), endothelial markers, were expressed to the same extent except for KDR, which is expressed more in EPDCs. They display a higher proliferation potential. Functional studies demonstrated that EPDCs were more sensitive to angiogenic factors, which afford these cells greater protection against cell death compared with HUVECs. Moreover, EPDCs exhibit more hematopoietic supportive activity than HUVECs. Finally, studies in nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice demonstrated that human circulating EPCs are able to colonize a Matrigel plug. EPDCs display the morphology and phenotype of endothelial cells. Their functional features indicate, however, that although these cells have undergone some differentiation steps, they still have the properties of immature cells, suggesting greater tissue repair capabilities. Future use of in vitro amplified peripheral blood EPDCs may constitute a challenging strategy for cell therapy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Madonna and R. De Caterina In vitro neovasculogenic potential of resident adipose tissue precursors Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1271 - C1280. [Abstract] [Full Text] [PDF] K. K. Hirschi, D. A. Ingram, and M. C. 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