SEARCH:   Advanced

Blood, 1 May 2004, Vol. 103, No. 9, pp. 3336-3341. Prepublished online as a Blood First Edition Paper on January 15, 2004; DOI 10.1182/blood-2003-06-1935. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-06-1935v1 103/9/3336    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kunisato, A. Articles by Hirai, H. Search for Related Content PubMed PubMed Citation Articles by Kunisato, A. Articles by Hirai, H. Related Collections Hematopoiesis and Stem Cells Gene Expression Free Research Articles Related Article in Blood Online Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Stem cell leukemia protein directs hematopoietic stem cell fate Atsushi Kunisato, Shigeru Chiba, Toshiki Saito, Keiki Kumano, Etsuko Nakagami-Yamaguchi, Tomoyuki Yamaguchi, and Hisamaru Hirai From the Departments of Hematology/Oncology and Cell Therapy/Transplantation Medicine, Graduate School of Medicine, University of Tokyo, Japan. Stem cell leukemia (SCL) protein has been shown to be an essential transcription factor during hematopoietic development in the embryo. In adult hematopoiesis, however, the role for SCL has remained largely unknown, whereas it is expressed in bone marrow hematopoietic stem cells (HSCs). In this study, we performed HSC transplantation and an in vitro HSC differentiation assay using retrovirally transduced HSCs with wild-type (WT) and dominant-negative (DN) SCL. The transplantation experiments showed that SCL does not affect the long-term repopulating capacity of HSCs but that WT SCL and DN SCL increase the short-term contribution of the transduced HSCs in myeloid and lymphoid lineages, respectively. An in vitro single-cell assay using a fetal thymus organ culture system further demonstrated that WT SCL facilitates HSCs to differentiate into the myeloid lineage but that DN SCL facilitates HSCs to differentiate into the lymphoid lineage. We conclude that the up-regulation or down-regulation of SCL directs HSCs toward myeloid or lymphoid lineage, respectively, although SCL does not affect their long-term repopulating capacity. (Blood. 2004;103: 3336-3341) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: The "stem cell leukemia" gene: what's in a name? Malcolm A. S. Moore Blood 2004 103: 3249-3250. [Full Text] [PDF] Related Letter in Blood Online: SCL/Tal1 and lymphoid versus myeloid lineage assignment Mark Hall, David Curtis, Atsushi Kunisato, Seishi Ogawa, and Shigeru Chiba Blood 2005 105: 1365-1366. [Full Text] [PDF] This article has been cited by other articles: M. T. Kassouf, H. Chagraoui, P. Vyas, and C. Porcher Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis Blood, August 15, 2008; 112(4): 1056 - 1067. [Abstract] [Full Text] [PDF] P. Brunet de la Grange, E. Zink, F. Armstrong, M.-C. Rouyez, and F. Pflumio Impairment of Granulo-Monocytic Development of Human Common Myeloid Progenitors but Not of Granulo-Monocytic Progenitors by Decreasing Stem Cell Leukemia/T-Cell Acute Leukemia 1 Expression Stem Cells, June 1, 2008; 26(6): 1658 - 1662. [Abstract] [Full Text] [PDF] L. Nie, H. Wu, and X.-H. Sun Ubiquitination and Degradation of Tal1/SCL Are Induced by Notch Signaling and Depend on Skp2 and CHIP J. Biol. Chem., January 11, 2008; 283(2): 684 - 692. [Abstract] [Full Text] [PDF] G. M. Crooks Another piece for the SCL/TAL1 puzzle Blood, November 1, 2006; 108(9): 2886 - 2886. [Full Text] [PDF] P. B. de la Grange, F. Armstrong, V. Duval, M.-C. Rouyez, N. Goardon, P.-H. Romeo, and F. Pflumio Low SCL/TAL1 expression reveals its major role in adult hematopoietic myeloid progenitors and stem cells Blood, November 1, 2006; 108(9): 2998 - 3004. [Abstract] [Full Text] [PDF] D. Reynaud, E. Ravet, M. Titeux, F. Mazurier, L. Renia, A. Dubart-Kupperschmitt, P.-H. Romeo, and F. Pflumio SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment Blood, October 1, 2005; 106(7): 2318 - 2328. [Abstract] [Full Text] [PDF] Y. Zhang, K. J. Payne, Y. Zhu, M. A. Price, Y. K. Parrish, E. Zielinska, L. W. Barsky, and G. M. Crooks SCL Expression at Critical Points in Human Hematopoietic Lineage Commitment Stem Cells, June 1, 2005; 23(6): 852 - 860. [Abstract] [Full Text] [PDF] M. Hall, D. Curtis, A. Kunisato, S. Ogawa, and S. Chiba SCL/Tal1 and lymphoid versus myeloid lineage assignment Blood, February 1, 2005; 105(3): 1365 - 1366. [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020