SEARCH:   Advanced

Blood, 1 July 2004, Vol. 104, No. 1, pp. 256-262. Prepublished online as a Blood First Edition Paper on March 18, 2004; DOI 10.1182/blood-2003-08-2661. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-08-2661v1 104/1/256    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Benasciutti, E. Articles by Crippa, M. P. Search for Related Content PubMed PubMed Citation Articles by Benasciutti, E. Articles by Crippa, M. P. Related Collections Hemostasis, Thrombosis, and Vascular Biology Neoplasia Signal Transduction Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  NEOPLASIA MAPK and JNK transduction pathways can phosphorylate Sp1 to activate the uPA minimal promoter element and endogenous gene transcription Elisa Benasciutti, Gilles Pagès, Olga Kenzior, William Folk, Francesco Blasi, and Massimo P. Crippa From the Laboratory of Molecular Genetics, S. Raffaele Scientific Institute and Università Vita-Salute S. Raffaele, Milano, Italy; Institute of Signaling, Developmental Biology, and Cancer Research, Centre Antoine Lacassagne, Nice, France; and Biochemistry Department, University of Missouri-Columbia. Two upstream regions of the human urokinase (uPA) gene regulate its transcription: the minimal promoter (MP) and the enhancer element. The activity of the minimal promoter is essential for basal uPA transcription in prostate adenocarcinoma PC3 cells. Binding of a phosphorylated Sp1 transcription factor is, in turn, essential for the activity of the MP. Here we report that the Jun kinase (JNK) pathway is required for the basal activity of the MP and for the expression of the endogenous uPA gene in PC3 cells and for activated transcription in LNCaP cells. On the other hand, the p42/p44 mitogen-activated protein kinase (MAPK) pathway activates uPA gene expression through Sp1 phosphorylation in HeLa, LNCaP, and CCL39-derivative cells that do not typically express uPA in basal conditions. In HeLa cells the dominant-negative form of JNK interferes with the p42/p44 MAPK activation of the uPA-MP. The results suggest that the stress-activated protein kinase (SAPK)/JNK pathway plays an important role in the phosphorylation of Sp1, which, in turn, leads to basal or activated transcription from the uPA-MP element. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. M. Park, D. I. Yule, and W. J. Bowers Tumor Necrosis Factor-{alpha}-mediated Regulation of the Inositol 1,4,5-Trisphosphate Receptor Promoter J. Biol. Chem., October 2, 2009; 284(40): 27557 - 27566. [Abstract] [Full Text] [PDF] S. Wei, H.-C. Chuang, W.-C. Tsai, H.-C. Yang, S.-R. Ho, A. J. Paterson, S. K. Kulp, and C.-S. Chen Thiazolidinediones Mimic Glucose Starvation in Facilitating Sp1 Degradation through the Up-Regulation of {beta}-Transducin Repeat-Containing Protein Mol. Pharmacol., July 1, 2009; 76(1): 47 - 57. [Abstract] [Full Text] [PDF] S.-Y. Gao, E.-M. Li, L. Cui, X.-F. Lu, L.-Y. Meng, H.-M. Yuan, J.-J. Xie, Z.-P. Du, J.-X. Pang, and L.-Y. Xu Sp1 and AP-1 Regulate Expression of the Human Gene VIL2 in Esophageal Carcinoma Cells J. Biol. Chem., March 20, 2009; 284(12): 7995 - 8004. [Abstract] [Full Text] [PDF] J.-Y. Chuang, Y.-T. Wang, S.-H. Yeh, Y.-W. Liu, W.-C. Chang, and J.-J. Hung Phosphorylation by c-Jun NH2-terminal Kinase 1 Regulates the Stability of Transcription Factor Sp1 during Mitosis Mol. Biol. Cell, March 1, 2008; 19(3): 1139 - 1151. [Abstract] [Full Text] [PDF] N. Y. Tan, V. C. Midgley, M. M. Kavurma, F. S. Santiago, X. Luo, R. Peden, R. G. Fahmy, M. C. Berndt, M. P. Molloy, and L. M. Khachigian Angiotensin II-Inducible Platelet-Derived Growth Factor-D Transcription Requires Specific Ser/Thr Residues in the Second Zinc Finger Region of Sp1 Circ. Res., February 29, 2008; 102(4): e38 - e51. [Abstract] [Full Text] [PDF] O. Norkina, A. Dolganiuc, T. Shapiro, K. Kodys, P. Mandrekar, and G. Szabo Acute alcohol activates STAT3, AP-1, and Sp-1 transcription factors via the family of Src kinases to promote IL-10 production in human monocytes J. Leukoc. Biol., September 1, 2007; 82(3): 752 - 762. [Abstract] [Full Text] [PDF] J. Choi, S. Y. Park, and C.-K. Joo Transforming Growth Factor-{beta}1 Represses E-Cadherin Production via Slug Expression in Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2708 - 2718. [Abstract] [Full Text] [PDF] S.-F. Chou, S.-W. Chang, and J.-L. Chuang Mitomycin C Upregulates IL-8 and MCP-1 Chemokine Expression via Mitogen-Activated Protein Kinases in Corneal Fibroblasts Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2009 - 2016. [Abstract] [Full Text] [PDF] C. Ferrai, D. Munari, P. Luraghi, L. Pecciarini, M. G. Cangi, C. Doglioni, F. Blasi, and M. P. Crippa A Transcription-dependent Micrococcal Nuclease-resistant Fragment of the Urokinase-type Plasminogen Activator Promoter Interacts with the Enhancer J. Biol. Chem., April 27, 2007; 282(17): 12537 - 12546. [Abstract] [Full Text] [PDF] M. Kanai, D. Wei, Q. Li, Z. Jia, J. Ajani, X. Le, J. Yao, and K. Xie Loss of kruppel-like factor 4 expression contributes to sp1 overexpression and human gastric cancer development and progression. Clin. Cancer Res., November 1, 2006; 12(21): 6395 - 6402. [Abstract] [Full Text] [PDF] S. Chu and T. J. Ferro Identification of a hydrogen peroxide-induced PP1-JNK1-Sp1 signaling pathway for gene regulation Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L983 - L992. [Abstract] [Full Text] [PDF] Y. Zhang, M. Liao, and M. L. Dufau Phosphatidylinositol 3-Kinase/Protein Kinase C{zeta}-Induced Phosphorylation of Sp1 and p107 Repressor Release Have a Critical Role in Histone Deacetylase Inhibitor-Mediated Depression of Transcription of the Luteinizing Hormone Receptor Gene. Mol. Cell. Biol., September 1, 2006; 26(18): 6748 - 6761. [Abstract] [Full Text] [PDF] A. E. Bilsland, K. Stevenson, S. Atkinson, W. Kolch, and W. N. Keith Transcriptional Repression of Telomerase RNA Gene Expression by c-Jun-NH2-Kinase and Sp1/Sp3 Cancer Res., February 1, 2006; 66(3): 1363 - 1370. [Abstract] [Full Text] [PDF] C. Rizzi, M. P. Crippa, R. E. Jeeninga, B. Berkhout, F. Blasi, G. Poli, and M. Alfano Pertussis Toxin B-Oligomer Suppresses IL-6 Induced HIV-1 and Chemokine Expression in Chronically Infected U1 Cells via Inhibition of Activator Protein 1 J. Immunol., January 15, 2006; 176(2): 999 - 1006. [Abstract] [Full Text] [PDF] C. Osipo, K. Meeke, H. Liu, D. Cheng, S. Lim, A. Weichel, and V. C. Jordan Trastuzumab Therapy for Tamoxifen-Stimulated Endometrial Cancer Cancer Res., September 15, 2005; 65(18): 8504 - 8513. [Abstract] [Full Text] [PDF] J.-A. Lee, D.-C. Suh, J.-E. Kang, M.-H. Kim, H. Park, M.-N. Lee, J.-M. Kim, B.-N. Jeon, H.-E. Roh, M.-Y. Yu, et al. Transcriptional Activity of Sp1 Is Regulated by Molecular Interactions between the Zinc Finger DNA Binding Domain and the Inhibitory Domain with Corepressors, and This Interaction Is Modulated by MEK J. Biol. Chem., July 29, 2005; 280(30): 28061 - 28071. [Abstract] [Full Text] [PDF] A. Brendolan, E. Ferretti, V. Salsi, K. Moses, S. Quaggin, F. Blasi, M. L. Cleary, and L. Selleri A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny Development, July 1, 2005; 132(13): 3113 - 3126. [Abstract] [Full Text] [PDF] Y. Li, T. Minamino, O. Tsukamoto, T. Yujiri, Y. Shintani, K.-i. Okada, Y. Nagamachi, M. Fujita, A. Hirata, S. Sanada, et al. Ablation of MEK Kinase 1 Suppresses Intimal Hyperplasia by Impairing Smooth Muscle Cell Migration and Urokinase Plasminogen Activator Expression in a Mouse Blood-Flow Cessation Model Circulation, April 5, 2005; 111(13): 1672 - 1678. [Abstract] [Full Text] [PDF] J. Ye, D. Shedd, and G. Miller An Sp1 Response Element in the Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50 Promoter Mediates Lytic Cycle Induction by Butyrate J. Virol., February 1, 2005; 79(3): 1397 - 1408. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020