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Blood, 1 December 2004, Vol. 104, No. 12, pp. 3655-3663. Prepublished online as a Blood First Edition Paper on August 17, 2004; DOI 10.1182/blood-2004-02-0412. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-02-0412v1 104/12/3655    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Platzer, B. Articles by Strobl, H. Search for Related Content PubMed PubMed Citation Articles by Platzer, B. Articles by Strobl, H. Related Collections Immunobiology Phagocytes Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY RelB regulates human dendritic cell subset development by promoting monocyte intermediates Barbara Platzer, Almut Jörgl, Sabine Taschner, Bernhard Höcher, and Herbert Strobl From the Institute of Immunology, University of Vienna, Austria. In humans, epithelial Langerhans cells (LCs) and monocyte-derived/interstitial dendritic cells (DCs) constitute 2 myeloid DC sublineages. Molecular mechanisms involved in their development from common myeloid progenitors remain poorly defined. Here we demonstrate that the nuclear factor-B (NF-B) transcription factor RelB regulates the generation of monocytic CD14+CD11b+ precursors of interstitial DCs from human hematopoietic progenitors. RelB overexpression promoted, whereas endogenous RelB inhibition (by p100N) blocked, precursor cell development along this DC subset pathway. RelB inhibition specifically arrested precursor progression from CD14loCD11b- to CD14+CD11b+ stages. Precursors were still capable of LC and granulocyte differentiation but were defective in macrophage–colony-stimulating factor (M-CSF)–dependent monocyte/macrophage differentiation. RelB inhibition markedly differed from classical NF-B signaling inhibition because IB superrepressor (IB-SR), but not p100N, impaired LC/DC differentiation, DC adhesion, and progenitor cell proliferation. Although RelB up-regulation and nuclear translocation are regarded as hallmarks of human myeloid DC maturation, ectopic RelB overexpression failed to promote DC maturation. Our results suggest that RelB regulates human monopoiesis and monocyte-derived DC subset development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Gobel, S. Taschner, J. Jurkin, S. Konradi, C. Vaculik, S. Richter, D. Kneidinger, C. Muhlbacher, C. Bieglmayer, A. Elbe-Burger, et al. Reciprocal role of GATA-1 and vitamin D receptor in human myeloid dendritic cell differentiation Blood, October 29, 2009; 114(18): 3813 - 3821. [Abstract] [Full Text] [PDF] B. Platzer, S. Richter, D. Kneidinger, D. Waltenberger, M. Woisetschlager, and H. Strobl Aryl Hydrocarbon Receptor Activation Inhibits In Vitro Differentiation of Human Monocytes and Langerhans Dendritic Cells J. Immunol., July 1, 2009; 183(1): 66 - 74. [Abstract] [Full Text] [PDF] S. J. A. M. Santegoets, A. J. M. van den Eertwegh, A. A. van de Loosdrecht, R. J. Scheper, and T. D. de Gruijl Human dendritic cell line models for DC differentiation and clinical DC vaccination studies J. Leukoc. Biol., December 1, 2008; 84(6): 1364 - 1373. [Abstract] [Full Text] [PDF] S. Vaira, T. Johnson, A. C. Hirbe, M. Alhawagri, I. Anwisye, B. Sammut, J. O'Neal, W. Zou, K. N. Weilbaecher, R. Faccio, et al. RelB is the NF-{kappa}B subunit downstream of NIK responsible for osteoclast differentiation PNAS, March 11, 2008; 105(10): 3897 - 3902. [Abstract] [Full Text] [PDF] Z. U. A. Mollah, S. Pai, C. Moore, B. J. O'Sullivan, M. J. Harrison, J. Peng, K. Phillips, J. B. Prins, J. Cardinal, and R. Thomas Abnormal NF-{kappa}B Function Characterizes Human Type 1 Diabetes Dendritic Cells and Monocytes J. Immunol., March 1, 2008; 180(5): 3166 - 3175. [Abstract] [Full Text] [PDF] S. Taschner, C. Koesters, B. Platzer, A. Jorgl, W. Ellmeier, T. Benesch, and H. Strobl Down-regulation of RXR{alpha} expression is essential for neutrophil development from granulocyte/monocyte progenitors Blood, February 1, 2007; 109(3): 971 - 979. [Abstract] [Full Text] [PDF] A. Jorgl, B. Platzer, S. Taschner, L. X. Heinz, B. Hocher, P. M. Reisner, F. Gobel, and H. Strobl Human Langerhans-cell activation triggered in vitro by conditionally expressed MKK6 is counterregulated by the downstream effector RelB Blood, January 1, 2007; 109(1): 185 - 193. [Abstract] [Full Text] [PDF] S. J. A. M. Santegoets, A. J. Masterson, P. C. van der Sluis, S. M. Lougheed, D. M. Fluitsma, A. J. M. van den Eertwegh, H. M. Pinedo, R. J. Scheper, and T. D. de Gruijl A CD34+ human cell line model of myeloid dendritic cell differentiation: evidence for a CD14+CD11b+ Langerhans cell precursor J. Leukoc. Biol., December 1, 2006; 80(6): 1337 - 1344. [Abstract] [Full Text] [PDF] L. X. Heinz, B. Platzer, P. M. Reisner, A. Jorgl, S. Taschner, F. Gobel, and H. Strobl Differential involvement of PU.1 and Id2 downstream of TGF-beta1 during Langerhans-cell commitment Blood, February 15, 2006; 107(4): 1445 - 1453. [Abstract] [Full Text] [PDF] P. J. Cejas, L. M. Carlson, D. Kolonias, J. Zhang, I. Lindner, D. D. Billadeau, L. H. Boise, and K. P. Lee Regulation of RelB Expression during the Initiation of Dendritic Cell Differentiation Mol. Cell. Biol., September 1, 2005; 25(17): 7900 - 7916. [Abstract] [Full Text] [PDF]

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