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Blood, 15 August 2004, Vol. 104, No. 4, pp. 1183-1190. Prepublished online as a Blood First Edition Paper on May 6, 2004; DOI 10.1182/blood-2004-01-0104. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: 2004-01-0104v1 104/4/1183    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mantegazza, A. R. Articles by Mordoh, J. Search for Related Content PubMed PubMed Citation Articles by Mantegazza, A. R. Articles by Mordoh, J. Related Collections Immunobiology Phagocytes Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells Adriana R. Mantegazza, María Marcela Barrio, Sandrine Moutel, Laura Bover, Markus Weck, Peter Brossart, Jean-Luc Teillaud, and José Mordoh From the Fundación Instituto Leloir and the Centro de Investigaciones Oncológicas-Fundación Cáncer, Buenos Aires, Argentina; the Department of Internal Medicine II, Division of Hematology, Oncology and Immunology, University of Tübingen, Germany; and Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 255, Centre de Recherches Biomédicales des Cordeliers, Paris, France. We analyzed herein whether members of the tetraspanin superfamily are involved in human immature dendritic cell (DC) functions such as foreign antigen internalization, phagocytosis, and cell migration. We show that CD63, CD9, CD81, CD82, and CD151 are present in immature DCs. Whereas CD9 and CD81 are mostly expressed at the cell surface, CD63 and CD82 are also located in intracellular organelles. Complexes of monoclonal antibody (Mab) FC-5.01-CD63 or Fab-5.01-CD63 were rapidly translocated "outside-in" and followed the endocytic pathway through early endosomes and lysosomes, reaching major histocompatibility complex (MHC) class II-enriched compartments (MIICs) in less than one hour. Internalization of CD63 was also observed during Saccharomyces cerevisiae phagocytosis. Moreover, an association of CD63 with the -glycan receptor dectin-1 was observed. Mabs against CD9, CD63, CD81, and CD82 enhanced by 50% the migration induced by the chemokines macrophage inflammatory protein-5 (MIP-5) and MIP-1. Concomitantly, Mabs against CD63 and CD82 diminished the surface expression of CD29, CD11b, CD18, and 5 integrins. By immunoprecipitation experiments we found that CD63 associated with integrins CD11b and CD18. These results suggest that CD9, CD63, CD81, and CD82 could play a role in modulating the interactions between immature DCs and their environment, slowing their migratory ability. However, only CD63 would intervene in the internalization of complex antigens. (Blood. 2004; 104:1183-1190) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Zhang, J. Kotha, L. K. Jennings, and X. A. Zhang Tetraspanins and vascular functions Cardiovasc Res, July 1, 2009; 83(1): 7 - 15. [Abstract] [Full Text] [PDF] J. Schroder, R. Lullmann-Rauch, N. Himmerkus, I. 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