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Blood, 1 September 2004, Vol. 104, No. 5, pp. 1324-1326. Prepublished online as a Blood First Edition Paper on May 20, 2004; DOI 10.1182/blood-2004-02-0618. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-02-0618v1 104/5/1324    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Clark, A. J. Articles by Humbert, P. O. Search for Related Content PubMed PubMed Citation Articles by Clark, A. J. Articles by Humbert, P. O. Related Collections Hematopoiesis and Stem Cells Oncogenes and Tumor Suppressors Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Brief report Cell-intrinsic requirement for pRb in erythropoiesis Allison J. Clark, Kathryn M. Doyle, and Patrick O. Humbert From the Cell Cycle and Cancer Genetics Laboratory, Trescowthick Research Laboratories, Peter MacCallum Cancer Centre, East Melbourne, Australia; and the Department of Pathology, University of Melbourne, Parkville, Australia. Retinoblastoma (Rb) and family members have been implicated as key regulators of cell proliferation and differentiation. In particular, accumulated data have suggested that the Rb gene product pRb is an important controller of erythroid differentiation. However, current published data are conflicting as to whether the role of pRb in erythroid cells is cell intrinsic or non–cell intrinsic. Here, we have made use of an in vitro erythroid differentiation culture system to determine the cell-intrinsic requirement for pRb in erythroid differentiation. We demonstrate that the loss of pRb function in primary differentiating erythroid cells results in impaired cell cycle exit and terminal differentiation. Furthermore, we have used coculture experiments to establish that this requirement is cell intrinsic. Together, these data unequivocally demonstrate that pRb is required in a cell-intrinsic manner for erythroid differentiation and provide clarification as to its role in erythropoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. R. Tallack, J. R. Keys, P. O. Humbert, and A. C. Perkins EKLF/KLF1 Controls Cell Cycle Entry via Direct Regulation of E2f2 J. Biol. Chem., July 31, 2009; 284(31): 20966 - 20974. [Abstract] [Full Text] [PDF] A. M. Pilon, M. O. Arcasoy, H. K. Dressman, S. E. Vayda, Y. D. Maksimova, J. I. Sangerman, P. G. Gallagher, and D. M. Bodine Failure of Terminal Erythroid Differentiation in EKLF-Deficient Mice Is Associated with Cell Cycle Perturbation and Reduced Expression of E2F2 Mol. Cell. Biol., December 15, 2008; 28(24): 7394 - 7401. [Abstract] [Full Text] [PDF] S. D. Berman, T. L. Yuan, E. S. Miller, E. Y. Lee, A. Caron, and J. A. Lees The Retinoblastoma Protein Tumor Suppressor Is Important for Appropriate Osteoblast Differentiation and Bone Development Mol. Cancer Res., September 1, 2008; 6(9): 1440 - 1451. [Abstract] [Full Text] [PDF] J. A. Chasis and N. Mohandas Erythroblastic islands: niches for erythropoiesis Blood, August 1, 2008; 112(3): 470 - 478. [Abstract] [Full Text] [PDF] V. G. Sankaran, S. H. Orkin,, and C. R. Walkley Rb intrinsically promotes erythropoiesis by coupling cell cycle exit with mitochondrial biogenesis Genes & Dev., February 15, 2008; 22(4): 463 - 475. [Abstract] [Full Text] [PDF] D. Daria, M.-D. Filippi, E. S. 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Humbert E2f4 regulates fetal erythropoiesis through the promotion of cellular proliferation Blood, August 1, 2006; 108(3): 886 - 895. [Abstract] [Full Text] [PDF] C. R. Walkley and S. H. Orkin Rb is dispensable for self-renewal and multilineage differentiation of adult hematopoietic stem cells PNAS, June 13, 2006; 103(24): 9057 - 9062. [Abstract] [Full Text] [PDF] H. Sun, Y. Chang, B. Schweers, M. A. Dyer, X. Zhang, S. W. Hayward, and D. W. Goodrich An E2F Binding-Deficient Rb1 Protein Partially Rescues Developmental Defects Associated with Rb1 Nullizygosity Mol. Cell. Biol., February 15, 2006; 26(4): 1527 - 1537. [Abstract] [Full Text] [PDF] A. H. Schuh, A. J. Tipping, A. J. Clark, I. Hamlett, B. Guyot, F. J. Iborra, P. Rodriguez, J. Strouboulis, T. Enver, P. Vyas, et al. ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis Mol. Cell. Biol., December 1, 2005; 25(23): 10235 - 10250. [Abstract] [Full Text] [PDF] R. Ferreira, K. Ohneda, M. Yamamoto, and S. Philipsen GATA1 Function, a Paradigm for Transcription Factors in Hematopoiesis Mol. Cell. Biol., February 15, 2005; 25(4): 1215 - 1227. [Full Text] [PDF]

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