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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2801-2809. Prepublished online as a Blood First Edition Paper on July 15, 2004; DOI 10.1182/blood-2004-03-1193. This Article Full Text Full Text (PDF) Supplemental Videos All Versions of this Article: 2004-03-1193v1 104/9/2801    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gunzer, M. Articles by Grabbe, S. Search for Related Content PubMed PubMed Citation Articles by Gunzer, M. Articles by Grabbe, S. Related Collections Signal Transduction Immunobiology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY A spectrum of biophysical interaction modes between T cells and different antigen-presenting cells during priming in 3-D collagen and in vivo Matthias Gunzer, Carsten Weishaupt, Anja Hillmer, Yasmin Basoglu, Peter Friedl, Kurt E. Dittmar, Waldemar Kolanus, Georg Varga, and Stephan Grabbe From the German Research Centre for Biotechnology, Junior Research Group Immunodynamics, Braunschweig, Germany; the Department of Dermatology, University of Münster, Münster, Germany; the Department of Dermatology, University of Würzburg, Würzburg, Germany; and the Institute for Molecular Physiology and Developmental Biology, University of Bonn, Bonn, Germany. For activation T cells engage antigen-presenting cells (APCs) in lymphatic tissues. The contact duration and kinetics (static versus dynamic) vary considerably in different model systems; however, it is unclear whether T cells, APCs, or the environment are responsible for the observed discrepancies. Using 3-D collagen matrices as structural scaffold, we directly compared the kinetics of T-cell engagement and activation by functionally major APC types, ie, dendritic cells (DCs) and resting or activated B cells. Resting B cells engaged T cells in long-lived (several hours), adhesive, and leukocyte function-associated antigen-1 (LFA-1)-dependent conjugates in 3-D collagen as well as in intact lymph nodes in vivo. DCs and preactivated B cells, however, supported predominantly dynamic, short-lived (minutes), and sequential contacts to T cells that were dependent on high cytoskeletal activity of the APCs but could not be inhibited by anti-LFA-1 treatment. Naive T cells were most strongly activated by DCs and activated B cells, whereas resting B cells were 100-fold less efficient to induce T-cell proliferation. Thus, in the same 3-D environment, naive T cells respond with a spectrum of different interaction modes dependent on the type and activation state of the APCs. Thereby, more dynamic interaction kinetics is positively correlated with higher T-cell priming efficiency. (Blood. 2004;104: 2801-2809) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: New ways for lymphocytes to meet Michael L. Dustin Blood 2004 104: 2618-2619. [Full Text] [PDF] This article has been cited by other articles: C. J. Kroger, S. Amoah, and M. A. Alexander-Miller Cutting Edge: Dendritic Cells Prime a High Avidity CTL Response Independent of the Level of Presented Antigen J. Immunol., May 1, 2008; 180(9): 5784 - 5788. [Abstract] [Full Text] [PDF] S. Balkow, F. Krux, K. Loser, J. U. Becker, S. 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