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Blood, 15 May 2005, Vol. 105, No. 10, pp. 4120-4126. Prepublished online as a Blood First Edition Paper on February 3, 2005; DOI 10.1182/blood-2004-02-0586. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-02-0586v1 105/10/4120    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jiang, X.-X. Articles by Mao, N. Search for Related Content PubMed PubMed Citation Articles by Jiang, X.-X. Articles by Mao, N. Related Collections Immunobiology Phagocytes Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells Xiao-Xia Jiang, Yi Zhang, Bing Liu, Shuang-Xi Zhang, Ying Wu, Xiao-Dan Yu, and Ning Mao From the Department of Cell Biology, Institute of Basic Medical Sciences, Beijing, P. R. China. Mesenchymal stem cells (MSCs), in addition to their multilineage differentiation, have a direct immunosuppressive effect on T-cell proliferation in vitro. However, it is unclear whether they also modulate the immune system by acting on the very first step. In this investigation, we addressed the effects of human MSCs on the differentiation, maturation, and function of dendritic cells (DCs) derived from CD14+ monocytes in vitro. Upon induction with granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4), MSC coculture could strongly inhibit the initial differentiation of monocytes to DCs, but this effect is reversible. In particular, such suppression could be recapitulated with no intercellular contact at a higher MSC/monocyte ratio (1:10). Furthermore, mature DCs treated with MSCs were significantly reduced in the expression of CD83, suggesting their skew to immature status. Meanwhile, decreased expression of presentation molecules (HLA-DR and CD1a) and costimulatory molecules (CD80 and CD86) and down-regulated IL-12 secretion were also observed. In consistence, the allostimulatory ability of MSC-treated mature DCs on allogeneic T cells was impaired. In conclusion, our data suggested for the first time that human MSCs could suppress monocyte differentiation into DCs, the most potent antigen-presenting cells (APCs), thus indicating the versatile regulation of MSCs on the ultimate specific immune response. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Mazar, M. Thomas, L. Bezrukov, A. Chanturia, G. Pekkurnaz, S. Yin, S. A. Kuznetsov, P. G. Robey, and J. Zimmerberg Cytotoxicity Mediated by the Fas Ligand (FasL)-activated Apoptotic Pathway in Stem Cells J. Biol. Chem., August 14, 2009; 284(33): 22022 - 22028. [Abstract] [Full Text] [PDF] R. Wehner, D. Wehrum, M. Bornhauser, S. Zhao, K. Schakel, M. P. Bachmann, U. Platzbecker, G. Ehninger, E. P. Rieber, and M. Schmitz Mesenchymal stem cells efficiently inhibit the proinflammatory properties of 6-sulfo LacNAc dendritic cells Haematologica, August 1, 2009; 94(8): 1151 - 1156. 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