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Blood, 1 June 2005, Vol. 105, No. 11, pp. 4308-4313. Prepublished online as a Blood First Edition Paper on February 10, 2005; DOI 10.1182/blood-2004-09-3578. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-09-3578v1 105/11/4308    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Abonia, J. P. Articles by Gurish, M. F. Search for Related Content PubMed PubMed Citation Articles by Abonia, J. P. Articles by Gurish, M. F. Related Collections Chemokines, Cytokines, and Interleukins Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2 J. Pablo Abonia, K. Frank Austen, Barrett J. Rollins, Sunil K. Joshi, Richard A. Flavell, William A. Kuziel, Pandelakis A. Koni, and Michael F. Gurish From the Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH; the Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA; the Molecular Immunology Program, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA; Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT; and Autoimmune and Inflammatory Diseases, Protein Design Labs, Fremont, CA. Homing of mast cell progenitors (MCps) to the mouse small intestine involves the interaction of 47 integrin with mucosal addressin cellular adhesion molecule-1 (MAdCAM-1). We now demonstrate the dependence of this process on CXC chemokine receptor 2 (CXCR2) and vascular cell adhesion molecule-1 (VCAM-1) using null strains and mice sublethally irradiated and bone marrow (BM) reconstituted (SIBR) with wild-type or null BM or with wild-type BM followed by administration of blocking antibody. The intestinal MCp concentration in CXCR2-/- mice was reduced by 67%, but was unaltered in CC chemokine receptor 2-/- (CCR2-/-), CCR3-/-, or CCR5-/- mice. SIBR mice given CXCR2-/- BM had an intestinal MCp concentration that was 76% less than that in BALB/c BM reconstituted mice. Antibody blockade of VCAM-1 or of CXCR2 in SIBR mice reduced intestinal MCp reconstitution, and mice lacking endothelial VCAM-1 also had a marked reduction relative to wild-type mice. Finally, the half-life of intestinal MCps in wild-type mice was less than one week on the basis of a more than 50% reduction by administration of anti-47 integrin or anti-CXCR2. Thus, the establishment and maintenance of MCps in the small intestine is a dynamic process that requires expression of the 47 integrin and the -chemokine receptor CXCR2. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Hallgren, T. G. Jones, J. P. Abonia, W. Xing, A. Humbles, K. F. Austen, and M. F. Gurish Pulmonary CXCR2 regulates VCAM-1 and antigen-induced recruitment of mast cell progenitors PNAS, December 18, 2007; 104(51): 20478 - 20483. [Abstract] [Full Text] [PDF] C. L. Weller, S. J. Collington, A. Hartnell, D. M. Conroy, T. Kaise, J. E. Barker, M. S. Wilson, G. W. Taylor, P. J. Jose, and T. J. Williams Chemotactic action of prostaglandin E2 on mouse mast cells acting via the PGE2 receptor 3 PNAS, July 10, 2007; 104(28): 11712 - 11717. [Abstract] [Full Text] [PDF] Y. Taketomi, K. Sunaga, S. Tanaka, M. Nakamura, S. Arata, T. Okuda, T.-C. Moon, H.-W. Chang, Y. Sugimoto, K. Kokame, et al. Impaired Mast Cell Maturation and Degranulation and Attenuated Allergic Responses in Ndrg1-Deficient Mice J. Immunol., June 1, 2007; 178(11): 7042 - 7053. [Abstract] [Full Text] [PDF] T.A. Silva, G.P. Garlet, S.Y. Fukada, J.S. Silva, and F.Q. Cunha Chemokines in Oral Inflammatory Diseases: Apical Periodontitis and Periodontal Disease J. Dent. Res., April 1, 2007; 86(4): 306 - 319. [Abstract] [Full Text] [PDF] P. Alcaide, T. G. Jones, G. M. Lord, L. H. Glimcher, J. Hallgren, Y. Arinobu, K. Akashi, A. M. Paterson, M. A. Gurish, and F. W. Luscinskas Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue J. Exp. Med., February 19, 2007; 204(2): 431 - 439. [Abstract] [Full Text] [PDF] J. P. Abonia, J. Hallgren, T. Jones, T. Shi, Y. Xu, P. Koni, R. A. Flavell, J. A. Boyce, K. F. Austen, and M. F. Gurish Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung Blood, September 1, 2006; 108(5): 1588 - 1594. [Abstract] [Full Text] [PDF] Y. Arinobu, H. Iwasaki, M. F. Gurish, S.-i. Mizuno, H. Shigematsu, H. Ozawa, D. G. Tenen, K. F. Austen, and K. Akashi Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis PNAS, December 13, 2005; 102(50): 18105 - 18110. [Abstract] [Full Text] [PDF]

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