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Blood, 15 January 2005, Vol. 105, No. 2, pp. 847-854. Prepublished online as a Blood First Edition Paper on July 1, 2004; DOI 10.1182/blood-2003-08-2942. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-08-2942v1 105/2/847    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Slichter, S. J. Articles by Bolgiano, D. Search for Related Content PubMed PubMed Citation Articles by Slichter, S. J. Articles by Bolgiano, D. Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Transfusion Medicine Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE Evaluation of different methods of leukoreduction of donor platelets to prevent alloimmune platelet refractoriness and induce tolerance in a canine transfusion model Sherrill J. Slichter, Douglas Fish, V. Kraig Abrams, Lakshmi Gaur, Karen Nelson, and Doug Bolgiano From the Puget Sound Blood Center and University of Washington School of Medicine, Seattle, WA. The effectiveness of different methods of leukoreduction in preventing alloimmune platelet refractoriness was evaluated in a canine model. Platelets from a random donor dog were administered for up to 8 weeks or until platelet refractoriness. Standard (STD; unmodified) platelets were accepted by 14% of recipients (n = 7) compared with 14% for centrifuge leukoreduced (C-LR) platelets (n = 21) and 31% for filter leukoreduced (F-LR) platelets (n = 13; no significant differences). Surprisingly, using both F-LR and C-LR platelets was highly effective (87% acceptance, n = 15). Transfusing F-LR/C-LR red blood cells (n = 4) or F-LR/C-LR plasma (n = 4), along with F-LR/C-LR platelets, did not affect platelet acceptance (100% acceptance). Overall acceptance of F-LR/C-LR platelets was 91% (n = 23; P .05 versus STD, C-LR, or F-LR platelets). F-LR/C-LR transfusions also induced tolerance to subsequent STD platelet transfusions from the same donor (82% acceptance, n = 19) as well as to donor skin grafts without recipient immunosuppression (57% acceptance, n = 7). To evaluate mechanisms of tolerance induction, F-LR/C-LR platelets were -irradiated. Although the -irradiated F-LR/C-LR platelets were uniformly accepted (n = 6), tolerance to STD platelets was lost. These data suggest that some allostimulatory white cells are filter adherent, whereas others escape filtration but can be removed by centrifugation and tolerance requires a residual functioning white cell. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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