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 Blood, 1 March 2005, Vol. 105, No. 5, pp. 1923-1929.
Prepublished online as a Blood First Edition Paper on November 12, 2004; DOI 10.1182/blood-2004-04-1450.

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HEMATOPOIESIS

PKC{delta} plays opposite roles in growth mediated by wild-type Kit and an oncogenic Kit mutant

Tanya Jelacic, and Diana Linnekin

From the Basic Research Laboratory, Center for Cancer Research, National Cancer Institute (NCI)–Frederick, Frederick, MD.

The Kit receptor tyrosine kinase is critical for normal hematopoiesis. Mutation of the aspartic acid residue encoded by codon 816 of human c-kit or codon 814 of the murine gene results in an oncogenic form of Kit. Here we investigate the role of protein kinase C{delta} (PKC{delta}) in responses mediated by wild-type murine Kit and the D814Y mutant in a murine mast cell-like line. PKC{delta} is activated after wild-type (WT) Kit binds stem cell factor (SCF), is constitutively active in cells expressing the Kit catalytic domain mutant, and coprecipitates with both forms of Kit. Inhibition of PKC{delta} had opposite effects on growth mediated by wild-type and mutant Kit. Both rottlerin and a dominant-negative PKC{delta} construct inhibited the growth of cells expressing mutant Kit, while SCF-induced growth of cells expressing wild-type Kit was not inhibited. Further, overexpression of PKC{delta} inhibited growth of cells expressing wild-type Kit and enhanced growth of cells expressing the Kit mutant. These data demonstrate that PKC{delta} contributes to factor-independent growth of cells expressing the D814Y mutant, but negatively regulates SCF-induced growth of cells expressing wild-type Kit. This is the first demonstration that PKC{delta} has different functions in cells expressing normal versus oncogenic forms of a receptor.


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