|
|
Blood, 1 March 2005, Vol. 105, No. 5, pp. 2168-2174.
Prepublished online as a Blood First Edition Paper on November 4, 2004; DOI 10.1182/blood-2004-02-0616.
 Previous Article | Table of Contents | Next Article 
RED CELLS
In vitro maturation of nascent reticulocytes to erythrocytes
Mark J. Koury,
Stephen T. Koury,
Prapaporn Kopsombut, and
Maurice C. Bondurant
From the Tennessee Valley Healthcare System Veterans Affairs Medical Center and Division of Hematology/Oncology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN; and the Department of Biotechnical and Clinical Laboratory Sciences, University of Buffalo, Buffalo, NY.
Most studies of mammalian reticulocyte maturation have used blood reticulocytes.Nascent reticulocytes, as found in bone marrow, have not been available in developmentally synchronized populations. Nascent murine reticulocytes formed in vitro by enucleation of Friend virus–infected erythroblasts were purified and recultured for 110 hours. At 0 hours, all recultured cells were lobulated and contained dense, centralized reticulin. By 110 hours, about 20% to 25% of the cells became biconcave erythrocytes. Most ribosomes and cellular RNAs were degraded within 20 hours, and during that period, heme synthesis declined from a rate equal to that of late erythroblasts to less than 10% of that rate. Many mitochondria appeared normal until they showed outer membrane swelling, degradation, and apparent fusion with intracellular vacuoles at 40 hours of culture. During the period of mitochondrial loss, Bcl-XL, an antiapoptotic protein that accumulates during erythroblast differentiation and maintains mitochondrial membrane integrity, demonstrated progressive decreases and changes consistent with deamidation. Nevertheless, the reticulocytes did not undergo apoptosis, because their apoptotic machinery was degraded. This experimental system that provides a developmentally synchronized population of nascent murine reticulocytes that mature into biconcave erythrocytes in vitro should be useful in further investigations of the cellular events involved in reticulocyte maturation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Zhang, M. S. Randall, M. R. Loyd, F. C. Dorsey, M. Kundu, J. L. Cleveland, and P. A. Ney
Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation
Blood,
July 2, 2009;
114(1):
157 - 164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Naarmann, C. Harnisch, N. Flach, E. Kremmer, H. Kuhn, D. H. Ostareck, and A. Ostareck-Lederer
mRNA Silencing in Human Erythroid Cell Maturation: HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K CONTROLS THE EXPRESSION OF ITS REGULATOR c-Src
J. Biol. Chem.,
June 27, 2008;
283(26):
18461 - 18472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. McGrath, P. D. Kingsley, A. D. Koniski, R. L. Porter, T. P. Bushnell, and J. Palis
Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus
Blood,
February 15, 2008;
111(4):
2409 - 2417.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-H. Goh, M. Josleyn, Y. T. Lee, R. L. Danner, R. B. Gherman, M. C. Cam, and J. L. Miller
The human reticulocyte transcriptome
Physiol Genomics,
July 18, 2007;
30(2):
172 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Su, J. M. May, M. J. Koury, and H. Asard
Human Erythrocyte Membranes Contain a Cytochrome b561 That May Be Involved in Extracellular Ascorbate Recycling
J. Biol. Chem.,
December 29, 2006;
281(52):
39852 - 39859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Rhodes, P. Kopsombut, M. C. Bondurant, J. O. Price, and M. J. Koury
Bcl-xL prevents apoptosis of late-stage erythroblasts but does not mediate the antiapoptotic effect of erythropoietin
Blood,
September 1, 2005;
106(5):
1857 - 1863.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
