|
|
Blood, 15 April 2005, Vol. 105, No. 8, pp. 3169-3177.
Prepublished online as a Blood First Edition Paper on January 4, 2005; DOI 10.1182/blood-2004-02-0452.
 Previous Article | Table of Contents | Next Article 
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1–dependent activation of PI-3K/Akt and regulation of Bcl-2 family members
Vidya Limaye,
Xiaochun Li,
Chris Hahn,
Pu Xia,
Michael C. Berndt,
Mathew A. Vadas, and
Jennifer R. Gamble
From the Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, SA, Australia; University of Adelaide, Adelaide, SA, Australia; and Monash University, Melbourne, Australia.
Sphingosine-1-phosphate (S1P), the bioactive product of sphingosine kinase (SK) activation, is a survival factor for endothelial cells. The mechanism of SK-mediated survival was investigated in endothelial cells with moderately raised intracellular SK activity. Overexpression of SK mediated survival primarily through the activation of the phosphatidyl inositol 3-kinase (PI-3K)/protein kinase B (Akt/PKB) pathway and an associated up-regulation of the antiapoptotic protein B cell lymphoma gene 2 (Bcl-2) and down-regulation of the proapoptotic protein bisindolylmaleimide (Bcl-2 interacting mediator of cell death; Bim). In addition there was an up-regulation and dephosphorylation of the junctional molecule platelet endothelial cell adhesion molecule-1 (PECAM-1), which was obligatory for activation of the PI-3K/Akt pathway, for SK-induced cell survival, and for the changes in the apoptosis-related proteins. Thus, raised intracellular SK activity induced a molecule involved in cell–cell interactions to augment cell survival through a PI-3K/Akt–dependent pathway. This is distinct from the activation of both PI-3K/Akt and mitogen-activated protein kinase (MAPK) pathways seen with exogenously added S1P. Cells overexpressing SK showed enhanced survival under conditions of serum deprivation and absence of attachment to extracellular matrix, suggesting a role for SK in the regulation of vascular phenomena that occur under conditions of stress, such as angiogenesis and survival in unattached states, as would be required for a circulating endothelial cell.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. R. Gamble, W. Y. Sun, X. Li, C. N. Hahn, S. M. Pitson, M. A. Vadas, and C. S. Bonder
Sphingosine Kinase-1 Associates with Integrin {alpha}V{beta}3 to Mediate Endothelial Cell Survival
Am. J. Pathol.,
November 1, 2009;
175(5):
2217 - 2225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. K. Wacker, T. S. Park, and J. M. Gidday
Hypoxic Preconditioning-Induced Cerebral Ischemic Tolerance: Role of Microvascular Sphingosine Kinase 2
Stroke,
October 1, 2009;
40(10):
3342 - 3348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hayashi, H. Nakagami, Y. Takami, H. Koriyama, M. Mori, K. Tamai, J. Sun, K. Nagao, R. Morishita, and Y. Kaneda
FHL-2 Suppresses VEGF-Induced Phosphatidylinositol 3-Kinase/Akt Activation via Interaction With Sphingosine Kinase-1
Arterioscler Thromb Vasc Biol,
June 1, 2009;
29(6):
909 - 914.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Bergelin, T. Blom, J. Heikkila, C. Lof, C. Alam, S. Balthasar, J. P. Slotte, A. Hinkkanen, and K. Tornquist
Sphingosine Kinase as an Oncogene: Autocrine Sphingosine 1-Phoshate Modulates ML-1 Thyroid Carcinoma Cell Migration by a Mechanism Dependent on Protein Kinase C-{alpha} and ERK1/2
Endocrinology,
May 1, 2009;
150(5):
2055 - 2063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Wadgaonkar, V. Patel, N. Grinkina, C. Romano, J. Liu, Y. Zhao, S. Sammani, J. G. N. Garcia, and V. Natarajan
Differential regulation of sphingosine kinases 1 and 2 in lung injury
Am J Physiol Lung Cell Mol Physiol,
April 1, 2009;
296(4):
L603 - L613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Bonder, W. Y. Sun, T. Matthews, C. Cassano, X. Li, H. S. Ramshaw, S. M. Pitson, A. F. Lopez, P. T. Coates, R. L. Proia, et al.
Sphingosine kinase regulates the rate of endothelial progenitor cell differentiation
Blood,
February 26, 2009;
113(9):
2108 - 2117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Yan, S. Chen, B. You, and J. Sun
Activation of sphingosine kinase-1 mediates induction of endothelial cell proliferation and angiogenesis by epoxyeicosatrienoic acids
Cardiovasc Res,
May 1, 2008;
78(2):
308 - 314.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Bergom, C. Paddock, C. Gao, T. Holyst, D. K. Newman, and P. J. Newman
An alternatively spliced isoform of PECAM-1 is expressed at high levels in human and murine tissues, and suggests a novel role for the C-terminus of PECAM-1 in cytoprotective signaling
J. Cell Sci.,
April 15, 2008;
121(8):
1235 - 1242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Li, M. Stankovic, C. S. Bonder, C. N. Hahn, M. Parsons, S. M. Pitson, P. Xia, R. L. Proia, M. A. Vadas, and J. R. Gamble
Basal and angiopoietin-1-mediated endothelial permeability is regulated by sphingosine kinase-1
Blood,
April 1, 2008;
111(7):
3489 - 3497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nakhaei-Nejad, A. M. Hussain, Q.-X. Zhang, and A. G. Murray
Endothelial PI 3-kinase activity regulates lymphocyte diapedesis
Am J Physiol Heart Circ Physiol,
December 1, 2007;
293(6):
H3608 - H3616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Q. Jin, J. Zhang, Y. Huang, H. E. Hoover, D. A. Vessey, and J. S. Karliner
A sphingosine kinase 1 mutation sensitizes the myocardium to ischemia/reperfusion injury
Cardiovasc Res,
October 1, 2007;
76(1):
41 - 50.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Affara, B. Dunmore, C. Savoie, S. Imoto, Y. Tamada, H. Araki, D. S. Charnock-Jones, S. Miyano, and C. Print
Understanding endothelial cell apoptosis: what can the transcriptome, glycome and proteome reveal?
Phil Trans R Soc B,
August 29, 2007;
362(1484):
1469 - 1487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhang, T. S. Park, and J. M. Gidday
Hypoxic preconditioning protects human brain endothelium from ischemic apoptosis by Akt-dependent survivin activation
Am J Physiol Heart Circ Physiol,
June 1, 2007;
292(6):
H2573 - H2581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhao, S. K. Kalari, P. V. Usatyuk, I. Gorshkova, D. He, T. Watkins, D. N. Brindley, C. Sun, R. Bittman, J. G. N. Garcia, et al.
Intracellular Generation of Sphingosine 1-Phosphate in Human Lung Endothelial Cells: ROLE OF LIPID PHOSPHATE PHOSPHATASE-1 AND SPHINGOSINE KINASE 1
J. Biol. Chem.,
May 11, 2007;
282(19):
14165 - 14177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tao, J. Zhang, D. A. Vessey, N. Honbo, and J. S. Karliner
Deletion of the Sphingosine Kinase-1 gene influences cell fate during hypoxia and glucose deprivation in adult mouse cardiomyocytes
Cardiovasc Res,
April 1, 2007;
74(1):
56 - 63.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Q. Jin and J. S. Karliner
Low dose N, N-dimethylsphingosine is cardioprotective and activates cytosolic sphingosine kinase by a PKC{varepsilon} dependent mechanism
Cardiovasc Res,
September 1, 2006;
71(4):
725 - 734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. De Palma, E. Meacci, C. Perrotta, P. Bruni, and E. Clementi
Endothelial Nitric Oxide Synthase Activation by Tumor Necrosis Factor {alpha} Through Neutral Sphingomyelinase 2, Sphingosine Kinase 1, and Sphingosine 1 Phosphate Receptors: A Novel Pathway Relevant to the Pathophysiology of Endothelium
Arterioscler Thromb Vasc Biol,
January 1, 2006;
26(1):
99 - 105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-S. Choi, J. T. Park, and J. S. Dumler
Anaplasma phagocytophilum Delay of Neutrophil Apoptosis through the p38 Mitogen-Activated Protein Kinase Signal Pathway
Infect. Immun.,
December 1, 2005;
73(12):
8209 - 8218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Chalfant and S. Spiegel
Sphingosine 1-phosphate and ceramide 1-phosphate: expanding roles in cell signaling
J. Cell Sci.,
October 15, 2005;
118(20):
4605 - 4612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
