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Blood, 1 May 2005, Vol. 105, No. 9, pp. 3633-3640. Prepublished online as a Blood First Edition Paper on January 11, 2005; DOI 10.1182/blood-2004-09-3740. This Article Full Text Full Text (PDF) All Versions of this Article: 2004-09-3740v1 105/9/3633    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Macallan, D. C. Articles by Beverley, P. C. L. Search for Related Content PubMed PubMed Citation Articles by Macallan, D. C. Articles by Beverley, P. C. L. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY B-cell kinetics in humans: rapid turnover of peripheral blood memory cells Derek C. Macallan, Diana L. Wallace, Yan Zhang, Hala Ghattas, Becca Asquith, Catherine de Lara, Andrew Worth, George Panayiotakopoulos, George E. Griffin, David F. Tough, and Peter C. L. Beverley From the Department of Cellular and Molecular Medicine–Infectious Diseases, St George's Hospital Medical School, Cranmer Terrace, London, United Kingdom; Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire, United Kingdom; Department of Immunology, Wright-Fleming Institute, Imperial College, London, United Kingdom. Information about the kinetic behavior and lifespan of lymphocytes is crucial to understanding the mechanisms that regulate processes such as immunologic memory. We have used in vivo labeling of dividing cells with 6,6-2H2-glucose, combined with cell sorting and gas-chromatography-mass spectrometry for deuterium enrichment, in order to analyze the kinetics of human total, naive, or memory B lymphocytes, separated from peripheral blood using monoclonal antibodies. We show that total blood B cells of young adults divide at an average rate of 1.9% (±1.0%) per day and at a similar though slightly slower rate, 1.5% (±1.3%) per day, in the elderly. Separation of naive and memory B cells according to expression of CD27 indicates that naive peripheral blood B cells divide slowly (0.46% per day), while memory cells proliferate more rapidly (2.66% per day). These data are compatible with the view that B-cell memory may be maintained by clones of proliferating B cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Ahuja, S. M. Anderson, A. Khalil, and M. J. Shlomchik Maintenance of the plasma cell pool is independent of memory B cells PNAS, March 25, 2008; 105(12): 4802 - 4807. [Abstract] [Full Text] [PDF] V. Hadinoto, M. Shapiro, T. C. Greenough, J. L. Sullivan, K. Luzuriaga, and D. A. Thorley-Lawson On the dynamics of acute EBV infection and the pathogenesis of infectious mononucleosis Blood, February 1, 2008; 111(3): 1420 - 1427. [Abstract] [Full Text] [PDF] B. Asquith, Y. Zhang, A. J. Mosley, C. M. de Lara, D. L. Wallace, A. Worth, L. Kaftantzi, K. Meekings, G. E. Griffin, Y. Tanaka, et al. In vivo T lymphocyte dynamics in humans and the impact of human T-lymphotropic virus 1 infection PNAS, May 8, 2007; 104(19): 8035 - 8040. [Abstract] [Full Text] [PDF] M. C. van Zelm, T. Szczepanski, M. van der Burg, and J. J.M. van Dongen Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion J. Exp. Med., March 19, 2007; 204(3): 645 - 655. [Abstract] [Full Text] [PDF]

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