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Blood, 1 December 2005, Vol. 106, No. 12, pp. 3979-3984. Prepublished online as a Blood First Edition Paper on August 4, 2005; DOI 10.1182/blood-2005-06-2398. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-06-2398v1 106/12/3979    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Delaby, C. Articles by Canonne-Hergaux, F. Search for Related Content PubMed PubMed Citation Articles by Delaby, C. Articles by Canonne-Hergaux, F. Related Collections Phagocytes Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES Presence of the iron exporter ferroportin at the plasma membrane of macrophages is enhanced by iron loading and down-regulated by hepcidin Constance Delaby, Nathalie Pilard, Ana Sofia Gonçalves, Carole Beaumont, and François Canonne-Hergaux From the Institut National de la Santé et de la Recherche Médicale (INSERM) U656, Fer et synthèse d'hème, Génétique, Physiologie et Pathologie, Faculté de médecine Xavier Bichat, Paris, France. Ferroportin, the only mammalian iron exporter identified to date, is highly expressed in duodenal enterocytes and in macrophages. Several lines of evidence indicate that in enterocytes the iron export mediated by ferroportin occurs and is regulated at the basolateral cell surface, where the transporter is strongly expressed. By contrast, in macrophages, ferroportin has been shown in intracellular vesicles. We used a high-affinity antibody to specify the localization of endogenous ferroportin expressed in primary culture of bone marrow–derived macrophages, in both basal and induced conditions. Our observations indicate that ferroportin is expressed in vesicular compartments that can reach the plasma membrane of macrophages. Of importance, when ferroportin expression was up-regulated through iron treatment or erythrophagocytosis, ferroportin expression was strongly enhanced at the plasma membrane of macrophages. Moreover, hepcidin dramatically reduced macrophage ferroportin protein levels. At the subcellular level, hepcidin was shown to induce rapid internalization and degradation of the macrophage iron exporter. These data are consistent with a direct iron export by ferroportin through the plasma membrane of macrophages and strongly support an efficient posttranscriptional down-regulation of ferroportin by hepcidin in these cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Chung, T. Chaston, J. Marks, S. K. Srai, and P. A. Sharp Hepcidin Decreases Iron Transporter Expression in Vivo in Mouse Duodenum and Spleen and in Vitro in THP-1 Macrophages and Intestinal Caco-2 Cells J. Nutr., August 1, 2009; 139(8): 1457 - 1462. [Abstract] [Full Text] [PDF] P. Robach, S. Recalcati, D. Girelli, C. Gelfi, N. J. Aachmann-Andersen, J. J. Thomsen, A. M. Norgaard, A. Alberghini, N. Campostrini, A. Castagna, et al. Alterations of systemic and muscle iron metabolism in human subjects treated with low-dose recombinant erythropoietin Blood, June 25, 2009; 113(26): 6707 - 6715. [Abstract] [Full Text] [PDF] P. Matak, T. B. 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