|
|
Blood, 15 August 2005, Vol. 106, No. 4, pp. 1479-1487.
Prepublished online as a Blood First Edition Paper on April 12, 2005; DOI 10.1182/blood-2004-11-4282.
 Previous Article | Table of Contents | Next Article 
TRANSPLANTATION
Effects of aging on the homing and engraftment of murine hematopoietic stem and progenitor cells
Ying Liang,
Gary Van Zant, and
Stephen J. Szilvassy
From the Departments of Physiology and Internal Medicine, University of Kentucky, Lexington, KY.
To test the hypothesis that aging has negative effects on stem-cell homing and engraftment, young or old C57BL/6 bone marrow (BM) cells were injected, using a limiting-dilution, competitive transplantation method, into old or young Ly5 congenic mice. Numbers of hematopoietic stem cells (HSCs) and progenitor cells (HPCs) recovered from BM or spleen were measured and compared with the numbers initially transplanted. Although the frequency of marrow competitive repopulation units (CRUs) increased approximately 2-fold from 2 months to 2 years of age, the BM homing efficiency of old CRUs was approximately 3-fold lower than that of young CRUs. Surprisingly, the overall size of individual stem-cell clones generated in recipients receiving a single CRU was not affected by donor age. However, the increased ages of HSC donors and HSC transplant recipients caused marked skewing of the pattern of engraftment toward the myeloid lineage, indicating that HSC-intrinsic and HSC-extrinsic (microenvironmental) age-related changes favor myelopoiesis. This correlated with changes after transplantation in the rate of recovery of circulating leukocytes, erythrocytes, and platelets. Recovery of the latter was especially blunted in aged recipients. Collectively, these findings may have implications for clinical HSC transplantation in which older persons increasingly serve as donors for elderly patients. (Blood. 2005; 106:1479-1487)
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. H. Cho, H. B. Sieburg, and C. E. Muller-Sieburg
A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells
Blood,
June 15, 2008;
111(12):
5553 - 5561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. P. Ertl, J. Chen, C. M. Astle, T. M. Duffy, and D. E. Harrison
Effects of dietary restriction on hematopoietic stem-cell aging are genetically regulated
Blood,
February 1, 2008;
111(3):
1709 - 1716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. J. Signer, E. Montecino-Rodriguez, O. N. Witte, J. McLaughlin, and K. Dorshkind
Age-related defects in B lymphopoiesis underlie the myeloid dominance of adult leukemia
Blood,
September 15, 2007;
110(6):
1831 - 1839.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. P. Zediak, I. Maillard, and A. Bhandoola
Multiple prethymic defects underlie age-related loss of T progenitor competence
Blood,
August 15, 2007;
110(4):
1161 - 1167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. N. Haylock, B. Williams, H. M. Johnston, M. C.P. Liu, K. E. Rutherford, G. A. Whitty, P. J. Simmons, I. Bertoncello, and S. K. Nilsson
Hemopoietic Stem Cells with Higher Hemopoietic Potential Reside at the Bone Marrow Endosteum
Stem Cells,
April 1, 2007;
25(4):
1062 - 1069.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Pearce, F. Anjos-Afonso, C. M. Ridler, A. Eddaoudi, and D. Bonnet
Age-Dependent Increase in Side Population Distribution Within Hematopoiesis: Implications for Our Understanding of the Mechanism of Aging
Stem Cells,
April 1, 2007;
25(4):
828 - 835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-G. Yao, F. M. Ellison, J. P. McCoy, J. Chen, and N. S. Young
Age-dependent accumulation of mtDNA mutations in murine hematopoietic stem cells is modulated by the nuclear genetic background
Hum. Mol. Genet.,
February 1, 2007;
16(3):
286 - 294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Ingram, T. R. Krier, L. E. Mead, C. McGuire, D. N. Prater, J. Bhavsar, M. R. Saadatzadeh, K. Bijangi-Vishehsaraei, F. Li, M. C. Yoder, et al.
Clonogenic Endothelial Progenitor Cells Are Sensitive to Oxidative Stress
Stem Cells,
February 1, 2007;
25(2):
297 - 304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Xing, M. A. Ryan, D. Daria, K. J. Nattamai, G. Van Zant, L. Wang, Y. Zheng, and H. Geiger
Increased hematopoietic stem cell mobilization in aged mice
Blood,
October 1, 2006;
108(7):
2190 - 2197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Kamminga and G. de Haan
Cellular Memory and Hematopoietic Stem Cell Aging
Stem Cells,
May 1, 2006;
24(5):
1143 - 1149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Van Zant
Stem cell markers: less is more!
Blood,
February 1, 2006;
107(3):
855 - 856.
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. H. Yilmaz, M. J. Kiel, and S. J. Morrison
SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity
Blood,
February 1, 2006;
107(3):
924 - 930.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
