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Blood, 1 September 2005, Vol. 106, No. 5, pp. 1601-1603. Prepublished online as a Blood First Edition Paper on May 24, 2005; DOI 10.1182/blood-2005-03-0987. This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 2005-03-0987v1 106/5/1601    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ng, E. S. Articles by Elefanty, A. G. Search for Related Content PubMed PubMed Citation Articles by Ng, E. S. Articles by Elefanty, A. G. Related Collections Hematopoiesis and Stem Cells Brief Reports Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Brief report Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation Elizabeth S. Ng, Richard P. Davis, Lisa Azzola, Edouard G. Stanley, and Andrew G. Elefanty From the Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia. To realize the therapeutic potential of human embryonic stem cells (hESCs), it is necessary to regulate their differentiation in a uniform and reproducible manner. We have developed a method in which known numbers of hESCs in serum-free medium were aggregated by centrifugation to foster the formation of embryoid bodies (EBs) of uniform size (spin EBs). These spin EBs differentiated efficiently and synchronously, as evidenced by the sequential expression of molecular markers representing stem cells, primitive streak, and mesoderm. In the presence of hematopoietic growth factors, reproducible differentiation was achieved with blood cells formed in more than 90% of EBs. Using chimeric EBs generated from mixtures of green fluorescence protein–positive (GFP+) and GFP– hESCs in a clonogenic assay, hematopoietic precursor frequency was estimated to be approximately 1:500 input cells. This method of EB formation provides a generally applicable means for modulating and objectively monitoring the directed differentiation of hESCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A new spin on hESC differentiation Manfred Baetscher Blood 2005 106: 1509. [Full Text] [PDF] This article has been cited by other articles: D. S. 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Stanley Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors Blood, February 15, 2008; 111(4): 1876 - 1884. [Abstract] [Full Text] [PDF] M. Bhatia Hematopoietic Development from Human Embryonic Stem Cells Hematology, January 1, 2007; 2007(1): 11 - 16. [Abstract] [Full Text] [PDF] M. A. Vodyanik, J. A. Thomson, and I. I. Slukvin Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures Blood, September 15, 2006; 108(6): 2095 - 2105. [Abstract] [Full Text] [PDF] K.-H. Chang, A. M. Nelson, H. Cao, L. Wang, B. Nakamoto, C. B. Ware, and T. Papayannopoulou Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin Blood, September 1, 2006; 108(5): 1515 - 1523. [Abstract] [Full Text] [PDF] A. Trounson The Production and Directed Differentiation of Human Embryonic Stem Cells Endocr. Rev., April 1, 2006; 27(2): 208 - 219. [Abstract] [Full Text] [PDF] M. W. Lensch and G. Q. Daley Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells Blood, April 1, 2006; 107(7): 2605 - 2612. [Abstract] [Full Text] [PDF]

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