SEARCH:   Advanced

Blood, 15 October 2005, Vol. 106, No. 8, pp. 2716-2722. Prepublished online as a Blood First Edition Paper on June 14, 2005; DOI 10.1182/blood-2005-01-0406. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-01-0406v1 106/8/2716    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Li, W. Articles by Esmon, C. T. Search for Related Content PubMed PubMed Citation Articles by Li, W. Articles by Esmon, C. T. Related Collections Gene Expression Hemostasis, Thrombosis, and Vascular Biology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Extraembryonic expression of EPCR is essential for embryonic viability Weihong Li, Xunzhen Zheng, Jian-Ming Gu, Gary L. Ferrell, Mingming Brady, Naomi L. Esmon, and Charles T. Esmon From the Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, the Howard Hughes Medical Institute, Oklahoma City, OK; Berlex Biosciences, Richmond, CA; and the Departments of Pathology and Biochemistry & Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK. The endothelial cell protein C receptor (EPCR) augments protein C activation by the thrombin-thrombomodulin complex. Deletion of the EPCR gene (Procr) in mice leads to embryonic lethality before embryonic day 10 (E10.0). EPCR is detected in the giant trophoblast cells at the feto-maternal boundary from E7.5 and weakly in embryonic aortic endothelial cells from E13.5, suggesting that extraembryonic EPCR expression may be essential for embryonic viability. Using conditional knock-out strategies, we demonstrate that Procr-deficient embryos with EPCR expression on placenta giant trophoblasts can be carried to term and then develop normally. Conversely, EPCR expression in the embryo, without expression in the giant trophoblast cells, does not rescue the mice. In genetically modified mice with low tissue factor activity, Procr deficiency is not lethal to the embryo. As adults, Procr-deficient mice generate more thrombin and activate less protein C in response to procoagulant stimuli. Spontaneous thrombin formation in the deficient animals increases with age. These findings show that extraembryonic EPCR expression is critical for embryo development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: It's the trophoblast! Hartmut Weiler Blood 2005 106: 2603. [Full Text] [PDF] This article has been cited by other articles: M. A. Rodger, M. Paidas, M. Claire, S. Middeldorp, S. Kahn, I. Martinelli, W. Hague, K. Rosene Montella, and I. Greer Inherited Thrombophilia and Pregnancy Complications Revisited Obstet. Gynecol., August 1, 2008; 112(2): 320 - 324. [Abstract] [Full Text] [PDF] M. Xue, L. March, P. N Sambrook, K. Fukudome, and C. J Jackson Endothelial protein C receptor is overexpressed in rheumatoid arthritic (RA) synovium and mediates the anti-inflammatory effects of activated protein C in RA monocytes Ann Rheum Dis, December 1, 2007; 66(12): 1574 - 1580. [Abstract] [Full Text] [PDF] R. Sood, M. Zogg, R. J. Westrick, Y.-h. Guo, E. J. Kerschen, G. Girardi, J. E. Salmon, S. R. Coughlin, and H. Weiler Fetal gene defects precipitate platelet-mediated pregnancy failure in factor V Leiden mothers J. Exp. Med., May 14, 2007; 204(5): 1049 - 1056. [Abstract] [Full Text] [PDF] M. Kaare, V.-M. Ulander, J.N. Painter, T. Ahvenainen, R. Kaaja, and K. Aittomaki Variations in the thrombomodulin and endothelial protein C receptor genes in couples with recurrent miscarriage Hum. Reprod., March 1, 2007; 22(3): 864 - 868. [Abstract] [Full Text] [PDF] X. Zheng, W. Li, J.-M. Gu, D. Qu, G. L. Ferrell, N. L. Esmon, and C. T. Esmon Effects of membrane and soluble EPCR on the hemostatic balance and endotoxemia in mice Blood, February 1, 2007; 109(3): 1003 - 1009. [Abstract] [Full Text] [PDF] J.-C. Gris Trophoblast cells sense maternal hemostasis Blood, April 15, 2006; 107(8): 3019 - 3020. [Full Text] [PDF] R. Sood, S. Kalloway, A. E. Mast, C. J. Hillard, and H. Weiler Fetomaternal cross talk in the placental vascular bed: control of coagulation by trophoblast cells Blood, April 15, 2006; 107(8): 3173 - 3180. [Abstract] [Full Text] [PDF] A. B. Balazs, A. J. Fabian, C. T. Esmon, and R. C. Mulligan Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow Blood, March 15, 2006; 107(6): 2317 - 2321. [Abstract] [Full Text] [PDF]

	Blood Online is supported in part by Genentech BioOncology and Biogen Idec
	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020