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Blood, 15 October 2005, Vol. 106, No. 8, pp. 2757-2760. Prepublished online as a Blood First Edition Paper on June 23, 2005; DOI 10.1182/blood-2005-03-1047. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-03-1047v1 106/8/2757    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Begonja, A. J. Articles by Walter, U. Search for Related Content PubMed PubMed Citation Articles by Begonja, A. J. Articles by Walter, U. Related Collections Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Signal Transduction Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Brief report Platelet NAD(P)H-oxidase–generated ROS production regulates IIb3-integrin activation independent of the NO/cGMP pathway Antonija Jurak Begonja, Stepan Gambaryan, Jörg Geiger, Barsom Aktas, Miroslava Pozgajova, Bernhard Nieswandt, and Ulrich Walter From the Institute of Clinical Biochemistry and Pathobiochemistry, University of Würzburg; and Rudolf Virchow Center, DFG Research Center for Experimental Biomedicine, University of Würzburg, Germany. Platelets play a crucial role in the physiology of primary hemostasis and pathophysiologic processes such as arterial thrombosis. Accumulating evidence suggests a role of reactive oxygen species (ROSs) in platelet activation. Here we show that platelets activated with different agonists produced intracellular ROSs, which were reduced by reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) oxidase inhibitors and superoxide scavengers. In addition, we demonstrate that ROSs produced in platelets significantly affected IIb3 integrin activation but not alpha and dense granule secretion and platelet shape change. Thrombin-induced integrin IIb3 activation was significantly decreased after pretreatment of platelets with NAD(P)H oxidase inhibitors (diphenylene iodonium [DPI] [45% ± 9%] and apocynin [43% ± 11%]) and superoxide scavengers (tiron [60% ± 9%] and Mn(III)tetrakis (1-methyl-4-pyridyl)porphyrin [MnTMPyP] [70% ± 6%]). These inhibitors also reduced platelet aggregation and thrombus formation on collagen under high shear and achieved their effects independent of the nitric oxide/cyclic guanosine monophosphate (NO/cGMP) pathway. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. E. Freedman Oxidative Stress and Platelets Arterioscler. Thromb. Vasc. Biol., March 1, 2008; 28(3): s11 - s16. [Abstract] [Full Text] [PDF] J. Zhou, Y. Chen, J.-Y. Lang, J.-J. Lu, and J. Ding Salvicine Inactivates {beta}1 Integrin and Inhibits Adhesion of MDA-MB-435 Cells to Fibronectin via Reactive Oxygen Species Signaling Mol. Cancer Res., February 1, 2008; 6(2): 194 - 204. [Abstract] [Full Text] [PDF] R. Priora, D. Summa, S. Frosali, A. Margaritis, D. Di Giuseppe, C. Lapucci, F. Ieri, F. M. Pulcinelli, A. Romani, F. Franconi, et al. Administration of Minor Polar Compound-Enriched Extra Virgin Olive Oil Decreases Platelet Aggregation and the Plasma Concentration of Reduced Homocysteine in Rats J. Nutr., January 1, 2008; 138(1): 36 - 41. [Abstract] [Full Text] [PDF] K. Bedard and K.-H. Krause The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology Physiol Rev, January 1, 2007; 87(1): 245 - 313. [Abstract] [Full Text] [PDF] K. Y. Stokes NAD(P)H oxidase: where there's smoke, there's fire Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H119 - H120. [Full Text] [PDF]

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