SEARCH:   Advanced

Blood, 1 January 2006, Vol. 107, No. 1, pp. 309-316. Prepublished online as a Blood First Edition Paper on July 28, 2005; DOI 10.1182/blood-2005-02-0666. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-02-0666v1 107/1/309    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Courret, N. Articles by Tardieux, I. Search for Related Content PubMed PubMed Citation Articles by Courret, N. Articles by Tardieux, I. Related Collections Phagocytes Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  PHAGOCYTES CD11c- and CD11b-expressing mouse leukocytes transport single Toxoplasma gondii tachyzoites to the brain Nathalie Courret, Sylvie Darche, Pierre Sonigo, Geneviève Milon, Dominique Buzoni-Gâtel, and Isabelle Tardieux From the Département des Maladies Infectieuses, Institut Cochin, Institut National de la Santé et de la Recherche Médicale (INSERM) U567–Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 8104; and Département de Parasitologie, Institut Pasteur, Paris, France. The protozoan parasite Toxoplasma gondii enters hosts through the intestinal mucosa and colonizes distant tissues such as the brain, where its progeny persists for a lifetime. We investigated the role of CD11c- and CD11b-expressing leukocytes in T gondii transport during the early step of parasitism from the mouse small intestine and during subsequent parasite localization in the brain. Following intragastric inoculation of cyst-containing parasites in mice, CD11c+ dendritic cells from the intestinal lamina propria, the Peyer patches, and the mesenteric lymph nodes were parasitized while in the blood, parasites were associated with the CD11c- CD11b+ monocytes. Using adoptive transfer experiments, we demonstrated that these parasitized cells triggered a parasitic process in the brain of naive recipient mice. Ex vivo analysis of parasitized leukocytes showed that single tachyzoites remained at the cell periphery, often surrounded by the host cell plasma membrane, but did not divide. Using either a dye that labels circulating leukocytes or an antibody known to prevent CD11b+ circulating leukocytes from leaving the microvascular bed lumen, and chimeric mice in which the hematopoietic cells expressed the green fluorescent protein, we established that T gondii zoites hijacked CD11b+ leukocytes to reach the brain extravascular space. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D O Zamora, J T Rosenbaum, and J R Smith Invasion of human retinal vascular endothelial cells by Toxoplasma gondii tachyzoites Br. J. Ophthalmol., June 1, 2008; 92(6): 852 - 855. [Abstract] [Full Text] [PDF] C. W. Lee, W. Sukhumavasi, and E. Y. Denkers Phosphoinositide-3-Kinase-Dependent, MyD88-Independent Induction of CC-Type Chemokines Characterizes the Macrophage Response to Toxoplasma gondii Strains with High Virulence Infect. Immun., December 1, 2007; 75(12): 5788 - 5797. [Abstract] [Full Text] [PDF] L. C. Menard, L. A. Minns, S. Darche, D. W. Mielcarz, D. M. Foureau, D. Roos, F. Dzierszinski, L. H. Kasper, and D. Buzoni-Gatel B Cells Amplify IFN-{gamma} Production By T Cells via a TNF-{alpha}-Mediated Mechanism J. Immunol., October 1, 2007; 179(7): 4857 - 4866. [Abstract] [Full Text] [PDF] V. B. Carruthers and Y. Suzuki Effects of Toxoplasma gondii Infection on the Brain Schizophr Bull, May 1, 2007; 33(3): 745 - 751. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020