SEARCH:   Advanced

Blood, 15 May 2006, Vol. 107, No. 10, pp. 3976-3982. Prepublished online as a Blood First Edition Paper on January 19, 2006; DOI 10.1182/blood-2005-11-4551. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-11-4551v1 107/10/3976    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Arnold, J. Articles by Green, P. L. Search for Related Content PubMed PubMed Citation Articles by Arnold, J. Articles by Green, P. L. Related Collections Hematopoiesis and Stem Cells Immunobiology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Enhancement of infectivity and persistence in vivo by HBZ, a natural antisense coded protein of HTLV-1 Joshua Arnold, Brenda Yamamoto, Min Li, Andrew J. Phipps, Ihab Younis, Michael D. Lairmore, and Patrick L. Green From the Departments of Veterinary Biosciences and Molecular Virology Immunology, and Medical Genetics, Center for Retrovirus Research, Comprehensive Cancer Center and Solove Research Institute, The Ohio State University, Columbus, OH. Natural antisense viral transcripts have been recognized in retroviruses, including human T-cell leukemia virus type 1 (HTLV-1), HIV-1, and feline immunodeficiency virus (FIV), and have been postulated to encode proteins important for the infection cycle and/or pathogenesis of the virus. The antisense strand of the HTLV-1 genome encodes HBZ, a novel nuclear basic region leucine zipper (b-ZIP) protein that in overexpression assays down-regulates Tax oncoprotein-induced viral transcription. Herein, we investigated the contribution of HBZ to HTLV-1–mediated immortalization of primary T lymphocytes in vitro and HTLV-1 infection in a rabbit animal model. HTLV-1 HBZ mutant viruses were generated and evaluated for viral gene expression, protein production, and immortalization capacity. Biologic properties of HBZ mutant viruses in vitro were indistinguishable from wild-type HTLV-1, providing the first direct evidence that HBZ is dispensable for viral replication and cellular immortalization. Rabbits inoculated with irradiated cells expressing HTLV-1 HBZ mutant viruses became persistently infected. However, these rabbits displayed a decreased antibody response to viral gene products and reduced proviral copies in peripheral blood mononuclear cells (PBMCs) as compared with wild-type HTLV-1–infected animals. Our findings indicated that HBZ was not required for in vitro cellular immortalization, but enhanced infectivity and persistence in inoculated rabbits. This study demonstrates that retroviruses use negative-strand–encoded proteins in the establishment of chronic viral infections. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: HTLV-1 HBZ: stop making sense Gerold Feuer Blood 2006 107: 3813-3814. [Full Text] [PDF] This article has been cited by other articles: M. Halin, E. Douceron, I. Clerc, C. Journo, N. L. Ko, S. Landry, E. L. Murphy, A. Gessain, I. Lemasson, J.-M. Mesnard, et al. Human T-cell leukemia virus type 2 produces a spliced antisense transcript encoding a protein that lacks a classic bZIP domain but still inhibits Tax2-mediated transcription Blood, September 17, 2009; 114(12): 2427 - 2438. [Abstract] [Full Text] [PDF] L. Xie, M. Kesic, B. Yamamoto, M. Li, I. Younis, M. D. Lairmore, and P. L. Green Human T-Cell Leukemia Virus Type 2 Rex Carboxy Terminus Is an Inhibitory/Stability Domain That Regulates Rex Functional Activity and Viral Replication J. Virol., May 15, 2009; 83(10): 5232 - 5243. [Abstract] [Full Text] [PDF] M. Li, M. Kesic, H. Yin, L. Yu, and P. L. Green Kinetic Analysis of Human T-Cell Leukemia Virus Type 1 Gene Expression in Cell Culture and Infected Animals J. Virol., April 15, 2009; 83(8): 3788 - 3797. [Abstract] [Full Text] [PDF] T. Zhao, J.-i. Yasunaga, Y. Satou, M. Nakao, M. Takahashi, M. Fujii, and M. Matsuoka Human T-cell leukemia virus type 1 bZIP factor selectively suppresses the classical pathway of NF-{kappa}B Blood, March 19, 2009; 113(12): 2755 - 2764. [Abstract] [Full Text] [PDF] J. Arnold, B. Zimmerman, M. Li, M. D. Lairmore, and P. L. Green Human T-cell leukemia virus type-1 antisense-encoded gene, Hbz, promotes T-lymphocyte proliferation Blood, November 1, 2008; 112(9): 3788 - 3797. [Abstract] [Full Text] [PDF] M. Yoshida, Y. Satou, J.-i. Yasunaga, J.-i. Fujisawa, and M. Matsuoka Transcriptional Control of Spliced and Unspliced Human T-Cell Leukemia Virus Type 1 bZIP Factor (HBZ) Gene J. Virol., October 1, 2008; 82(19): 9359 - 9368. [Abstract] [Full Text] [PDF] I. Clerc, N. Polakowski, C. Andre-Arpin, P. Cook, B. Barbeau, J.-M. Mesnard, and I. Lemasson An Interaction between the Human T Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor (HBZ) and the KIX Domain of p300/CBP Contributes to the Down-regulation of Tax-dependent Viral Transcription by HBZ J. Biol. Chem., August 29, 2008; 283(35): 23903 - 23913. [Abstract] [Full Text] [PDF] K. Pichler, T. Kattan, J. Gentzsch, A. K. Kress, G. P. Taylor, C. R. M. Bangham, and R. Grassmann Strong induction of 4-1BB, a growth and survival promoting costimulatory receptor, in HTLV-1-infected cultured and patients' T cells by the viral Tax oncoprotein Blood, May 1, 2008; 111(9): 4741 - 4751. [Abstract] [Full Text] [PDF] S. A. Chevalier, M. Walic, S. Calattini, A. Mallet, M.-C. Prevost, A. Gessain, and R. Mahieux Construction and Characterization of a Full-Length Infectious Simian T-Cell Lymphotropic Virus Type 3 Molecular Clone J. Virol., June 15, 2007; 81(12): 6276 - 6285. [Abstract] [Full Text] [PDF] M. Miyazaki, J.-I. Yasunaga, Y. Taniguchi, S. Tamiya, T. Nakahata, and M. Matsuoka Preferential Selection of Human T-Cell Leukemia Virus Type 1 Provirus Lacking the 5' Long Terminal Repeat during Oncogenesis J. Virol., June 1, 2007; 81(11): 5714 - 5723. [Abstract] [Full Text] [PDF] I. Lemasson, M. R. Lewis, N. Polakowski, P. Hivin, M.-H. Cavanagh, S. Thebault, B. Barbeau, J. K. Nyborg, and J.-M. Mesnard Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Protein Interacts with the Cellular Transcription Factor CREB To Inhibit HTLV-1 Transcription J. Virol., February 15, 2007; 81(4): 1543 - 1553. [Abstract] [Full Text] [PDF] J.-M. Mesnard, B. Barbeau, and C. Devaux HBZ, a new important player in the mystery of adult T-cell leukemia Blood, December 15, 2006; 108(13): 3979 - 3982. [Abstract] [Full Text] [PDF] P. Miyazato, J.-i. Yasunaga, Y. Taniguchi, Y. Koyanagi, H. Mitsuya, and M. Matsuoka De Novo Human T-Cell Leukemia Virus Type 1 Infection of Human Lymphocytes in NOD-SCID, Common {gamma}-Chain Knockout Mice J. Virol., November 1, 2006; 80(21): 10683 - 10691. [Abstract] [Full Text] [PDF] S. Calattini, S. A. Chevalier, R. Duprez, P. Afonso, A. Froment, A. Gessain, and R. Mahieux Human T-Cell Lymphotropic Virus Type 3: Complete Nucleotide Sequence and Characterization of the Human Tax3 Protein J. Virol., October 1, 2006; 80(19): 9876 - 9888. [Abstract] [Full Text] [PDF] W. M. Switzer, S. H. Qari, N. D. Wolfe, D. S. Burke, T. M. Folks, and W. Heneine Ancient origin and molecular features of the novel human T-lymphotropic virus type 3 revealed by complete genome analysis. J. Virol., August 1, 2006; 80(15): 7427 - 7438. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020