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Blood, 1 June 2006, Vol. 107, No. 11, pp. 4466-4474. Prepublished online as a Blood First Edition Paper on February 9, 2006; DOI 10.1182/blood-2005-08-3490. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-08-3490v1 107/11/4466    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Holl, V. Articles by Moog, C. Search for Related Content PubMed PubMed Citation Articles by Holl, V. Articles by Moog, C. Related Collections Immunobiology Phagocytes Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Efficient inhibition of HIV-1 replication in human immature monocyte-derived dendritic cells by purified anti–HIV-1 IgG without induction of maturation Vincent Holl, Maryse Peressin, Sylvie Schmidt, Thomas Decoville, Susan Zolla-Pazner, Anne-Marie Aubertin, and Christiane Moog From the Institut de Virologie, Strasbourg, France; and Veterans Affairs and New York University Medical Centers, NY. During mucosal HIV transmission, immature dendritic cells (DCs) present in the mucosa are among the first cellular targets of the virus. Previous studies have analyzed the inhibition of HIV-1 transfer from human mature DCs to T lymphocytes by neutralizing IgG, but so far no in vitro data regarding the capacity of antibodies to inhibit HIV-1 infection of immature DCs have been reported. Here, we found an increased HIV-inhibitory activity of monoclonal IgG and purified polyclonal IgG when immature monocyte-derived dendritic cells (iMDDCs) were used as target cells instead of autologous blood lymphocytes. We showed that FcRII is involved in the mechanism for inhibiting HIV-1 infection of iMDDCs by IgG, whereas no induction of maturation was detected at concentrations of IgG that result in a 90% reduction of HIV replication. After induction of FcRI expression on iMDDCs by IFN-, an augmentation of the HIV-inhibitory activity of IgG, related to the expression of FcRI, was observed. Taken together, our results demonstrate the participation of FcRs in HIV-1 inhibition by IgG when iMDDCs are the targets. We propose that IgG is able to efficiently inhibit HIV-1 replication in iMDDCs and should be one of the components to be induced by vaccination. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Wilflingseder, Z. Banki, E. Garcia, M. Pruenster, G. Pfister, B. Muellauer, D. S. Nikolic, C. Gassner, C. G. Ammann, M. P. Dierich, et al. IgG Opsonization of HIV Impedes Provirus Formation in and Infection of Dendritic Cells and Subsequent Long-Term Transfer to T Cells J. Immunol., June 15, 2007; 178(12): 7840 - 7848. [Abstract] [Full Text] [PDF] T. Demberg, R. H. Florese, M. J. Heath, K. Larsen, I. Kalisz, V. S. Kalyanaraman, E. M. Lee, R. Pal, D. Venzon, R. Grant, et al. A Replication-Competent Adenovirus-Human Immunodeficiency Virus (Ad-HIV) tat and Ad-HIV env Priming/Tat and Envelope Protein Boosting Regimen Elicits Enhanced Protective Efficacy against Simian/Human Immunodeficiency Virus SHIV89.6P Challenge in Rhesus Macaques J. Virol., April 1, 2007; 81(7): 3414 - 3427. [Abstract] [Full Text] [PDF] T. van Montfort, A. A. Nabatov, T. B. H. Geijtenbeek, G. Pollakis, and W. A. Paxton Efficient Capture of Antibody Neutralized HIV-1 by Cells Expressing DC-SIGN and Transfer to CD4+ T Lymphocytes J. Immunol., March 1, 2007; 178(5): 3177 - 3185. [Abstract] [Full Text] [PDF]

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