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Blood, 15 June 2006, Vol. 107, No. 12, pp. 4687-4694. Prepublished online as a Blood First Edition Paper on February 28, 2006; DOI 10.1182/blood-2005-12-4995. This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: 2005-12-4995v1 107/12/4687    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Unwin, R. D. Articles by Whetton, A. D. Search for Related Content PubMed PubMed Citation Articles by Unwin, R. D. Articles by Whetton, A. D. Related Collections Hematopoiesis and Stem Cells Gene Expression Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Quantitative proteomics reveals posttranslational control as a regulatory factor in primary hematopoietic stem cells Richard D. Unwin, Duncan L. Smith, David Blinco, Claire L. Wilson, Crispin J. Miller, Caroline A. Evans, Ewa Jaworska, Stephen A. Baldwin, Kay Barnes, Andrew Pierce, Elaine Spooncer, and Anthony D. Whetton From the Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Manchester; the Bioinformatics Group, Paterson Institute for Cancer Research, Christie Hospital, Manchester; and the Institute of Membrane and Systems Biology, Faculty of Biological Sciences, University of Leeds, United Kingdom. The proteome is determined by rates of transcription, translation, and protein turnover. Definition of stem cell populations therefore requires a stem cell proteome signature. However, the limit to the number of primary cells available has restricted extensive proteomic analysis. We present a mass spectrometric method using an isobaric covalent modification of peptides for relative quantification (iTRAQ), which was employed to compare the proteomes of approximately 1 million long-term reconstituting hematopoietic stem cells (Lin–Sca+Kit+; LSK+) and non–long-term reconstituting progenitor cells (Lin–Sca+Kit–; LSK–), respectively. Extensive 2-dimensional liquid chromatography (LC) peptide separation prior to mass spectrometry (MS) enabled enhanced proteome coverage with relative quantification of 948 proteins. Of the 145 changes in the proteome, 54% were not seen in the transcriptome. Hypoxia-related changes in proteins controlling metabolism and oxidative protection were observed, indicating that LSK+ cells are adapted for anaerobic environments. This approach can define proteomic changes in primary samples, thereby characterizing the molecular signature of stem cells and their progeny. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: An anaerobic home for the stem cell proteome Kateri Moore Blood 2006 107: 4578. [Full Text] [PDF] This article has been cited by other articles: M. R. Saadatzadeh, K. Bijangi-Vishehsaraei, R. Kapur, and L. S. Haneline Distinct roles of stress-activated protein kinases in Fanconi anemia type C-deficient hematopoiesis Blood, March 19, 2009; 113(12): 2655 - 2660. [Abstract] [Full Text] [PDF] A. Pierce, R. D. Unwin, C. A. Evans, S. Griffiths, L. Carney, L. Zhang, E. Jaworska, C.-F. Lee, D. Blinco, M. J. Okoniewski, et al. Eight-channel iTRAQ Enables Comparison of the Activity of Six Leukemogenic Tyrosine Kinases Mol. Cell. Proteomics, May 1, 2008; 7(5): 853 - 863. [Abstract] [Full Text] [PDF] A. J. K. Williamson, D. L. Smith, D. 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[Abstract] [Full Text] [PDF] E. Grinstein, Y. Du, S. Santourlidis, J. Christ, M. Uhrberg, and P. Wernet Nucleolin Regulates Gene Expression in CD34-positive Hematopoietic Cells J. Biol. Chem., April 27, 2007; 282(17): 12439 - 12449. [Abstract] [Full Text] [PDF] K. Parmar, P. Mauch, J.-A. Vergilio, R. Sackstein, and J. D. Down Distribution of hematopoietic stem cells in the bone marrow according to regional hypoxia PNAS, March 27, 2007; 104(13): 5431 - 5436. [Abstract] [Full Text] [PDF] R. W. Georgantas III, R. Hildreth, S. Morisot, J. Alder, C.-g. Liu, S. Heimfeld, G. A. Calin, C. M. Croce, and C. I. Civin CD34+ hematopoietic stem-progenitor cell microRNA expression and function: A circuit diagram of differentiation control PNAS, February 20, 2007; 104(8): 2750 - 2755. [Abstract] [Full Text] [PDF]

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