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Blood, 1 February 2006, Vol. 107, No. 3, pp. 924-930. Prepublished online as a Blood First Edition Paper on October 11, 2005; DOI 10.1182/blood-2005-05-2140. This Article Full Text Full Text (PDF) All Versions of this Article: 2005-05-2140v1 107/3/924    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yilmaz, O. H. Articles by Morrison, S. J. Search for Related Content PubMed PubMed Citation Articles by Yilmaz, O. H. Articles by Morrison, S. J. Related Collections Hematopoiesis and Stem Cells Stem Cells in Hematology Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity Ömer H. Yilmaz, Mark J. Kiel, and Sean J. Morrison From the Howard Hughes Medical Institute, Life Sciences Institute, Department of Internal Medicine, and Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI. Recent advances have increased the purity of hematopoietic stem cells (HSCs) isolated from young mouse bone marrow. However, little attention has been paid to the purity of HSCs from other contexts. Although Thy-1lowSca-1+Lineage-c-kit+ cells from young bone marrow are highly enriched for HSCs (1 in 5 cells gives long-term multilineage reconstitution after transplantation into irradiated mice), the same population from old, reconstituted, or cytokine-mobilized mice engrafts much less efficiently (1 in 78 to 1 in 185 cells gives long-term multilineage reconstitution). To test whether we could increase the purity of HSCs isolated from these contexts, we examined the SLAM family markers CD150 and CD48. All detectable HSCs from old, reconstituted, and cyclophosphamide/G-CSF-mobilized mice were CD150+CD48-, just as in normal young bone marrow. Thy-1lowSca-1+Lineage-c-kit+ cells from old, reconstituted, or mobilized mice included mainly CD48+ and/or CD150- cells that lacked reconstituting ability. CD150+CD48-Sca-1+Lineage-c-kit+ cells from old, reconstituted, or mobilized mice were much more highly enriched for HSCs, with 1 in 3 to 1 in 7 cells giving long-term multilineage reconstitution. SLAM family receptor expression is conserved among HSCs from diverse contexts, and HSCs from old, reconstituted, and mobilized mice engraft relatively efficiently after transplantation when contaminating cells are eliminated. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Stem cell markers: less is more! Gary Van Zant Blood 2006 107: 855-856. [Full Text] [PDF] This article has been cited by other articles: X. Hu, H. Shen, C. Tian, H. Yu, G. Zheng, R. XuFeng, Z. Ju, J. Xu, J. Wang, and T. Cheng Kinetics of normal hematopoietic stem and progenitor cells in a Notch1-induced leukemia model Blood, October 29, 2009; 114(18): 3783 - 3792. [Abstract] [Full Text] [PDF] R. XuFeng, M. J. Boyer, H. Shen, Y. Li, H. Yu, Y. Gao, Q. 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