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Blood, 15 February 2006, Vol. 107, No. 4, pp. 1665-1672. Prepublished online as a Blood First Edition Paper on November 17, 2005; DOI 10.1182/blood-2005-08-3097. This Article Full Text Full Text (PDF) Supplemental Table and Figures All Versions of this Article: 2005-08-3097v1 2005-08-3097v2 107/4/1665    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kingsley, P. D. Articles by Palis, J. Search for Related Content PubMed PubMed Citation Articles by Kingsley, P. D. Articles by Palis, J. Related Collections Hematopoiesis and Stem Cells Red Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  RED CELLS "Maturational" globin switching in primary primitive erythroid cells Paul D. Kingsley, Jeffrey Malik, Rachel L. Emerson, Timothy P. Bushnell, Kathleen E. McGrath, Laura A. Bloedorn, Michael Bulger, and James Palis From the Departments of Pediatrics and Biochemistry/Biophysics, University of Rochester Medical Center, Center for Pediatric Biomedical Research, Rochester, NY. Mammals have 2 distinct erythroid lineages. The primitive erythroid lineage originates in the yolk sac and generates a cohort of large erythroblasts that terminally differentiate in the bloodstream. The definitive erythroid lineage generates smaller enucleated erythrocytes that become the predominant cell in fetal and postnatal circulation. These lineages also have distinct globin expression patterns. Our studies in primary murine primitive erythroid cells indicate that H1 is the predominant -globin transcript in the early yolk sac. Thus, unlike the human, murine -globin genes are not up-regulated in the order of their chromosomal arrangement. As primitive erythroblasts mature from proerythroblasts to reticulocytes, they undergo a H1- to y-globin switch, up-regulate adult 1- and 2-globins, and down-regulate -globin. These changes in transcript levels correlate with changes in RNA polymerase II density at their promoters and transcribed regions. Furthermore, the y- and H1-globin genes in primitive erythroblasts reside within a single large hyperacetylated domain. These data suggest that this "maturational" H1- to y-globin switch is dynamically regulated at the transcriptional level. Globin switching during ontogeny is due not only to the sequential appearance of primitive and definitive lineages but also to changes in globin expression as primitive erythroblasts mature in the bloodstream. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C.-P. Chaturvedi, A. M. Hosey, C. Palii, C. Perez-Iratxeta, Y. Nakatani, J. A. Ranish, F. J. Dilworth, and M. Brand Dual role for the methyltransferase G9a in the maintenance of {beta}-globin gene transcription in adult erythroid cells PNAS, October 27, 2009; 106(43): 18303 - 18308. [Abstract] [Full Text] [PDF] G. Fromm, C. de Vries, R. Byron, J. Fields, S. Fiering, M. Groudine, M. A. Bender, J. Palis, and M. Bulger Histone hyperacetylation within the {beta}-globin locus is context-dependent and precedes high-level gene expression Blood, October 15, 2009; 114(16): 3479 - 3488. [Abstract] [Full Text] [PDF] S. T. Chou, E. Khandros, L. C. Bailey, K. E. 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